1. Recombinant Proteins
  2. Recombinant Human Insulin-like Growth Factor-Binding Protein 3

Recombinant Human Insulin-like Growth Factor-Binding Protein 3

Cat. No.: HY-P7020
Handling Instructions

Recombinant Human Insulin-like Growth Factor-Binding Protein 3 belongs to insulin-like growth factor-binding protein, prolongs the half-life of the insulin-like growth factors (IGFs), inhibits interaction of IGFs with their cell surface receptors, and functions in cancer reduction.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $160 In-stock
Estimated Time of Arrival: December 31
50 μg $360 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rHuIGF-BP3; IBP-3; IGF-binding protein 3; IGFBP-3
Species

Human

Source

E. coli

Accession

P17936

Gene ID

3486

Molecular Weight

Approximately 28.8 kDa

AA Sequence

GASSGGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGEVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKR

Biological Activity
The ED50 is <200 ng/mL as measured by serum free human MCF-7 cells, corresponding to a specific activity of >5.0 × 103 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Insulin-like Growth Factor-Binding Protein 3 (rHuIGF-BP3) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA.

Storage & Stability

Lyophilized recombinant Human Insulin-like Growth Factor-Binding Protein 3 (rHuIGF-BP3) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Insulin-like Growth Factor-Binding Proteins (IGFBPs) modulate the actions of secreted insulin-like growth facts (IGFs) by binding to them and increase the IGF halflife in the extracellular milieu and circulation by sequestering them in this bound form. IGFBPs either enhance or inhibit IGF actions on target cells; the individual IGFBPs either inhibit or potentiate IGF effects on osteoblasts in bone[1]. Insulin-like Growth Factor-Binding Protein 3 (IGFBP3) is involved in prolonging the half-life of the IGFs (from 12 minutes to 12 hours), inhibits interaction of IGFs with their cell surface receptors, as well as, it is a circulating reservoir of IGFs. Moreover, IGFBP3 functions in cancer reduction, by both IGF-dependent and IGF-independent mechanisms. IGFBP3 has been shown to reduce the risk of breast cancer in high doses. A high concentration of IGFBP3 prevents IGF1:IGF1R interaction and blocks the IGF1R tyrosine kinase activity, resulting in the inhibition of the formation of intracellular signaling cascades[2].

Documentation
References
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Product Name:
Recombinant Human Insulin-like Growth Factor-Binding Protein 3
Cat. No.:
HY-P7020
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