1. Recombinant Proteins
  2. Recombinant Human Interleukin-1 alpha

Recombinant Human Interleukin-1 alpha

Cat. No.: HY-P7027
Handling Instructions

Recombinant Human Interleukin-1 alpha is a polypeptidic cytokine that play a key role in inflammation and immunologic reactions.

For research use only. We do not sell to patients.

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Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $220 In-stock
Estimated Time of Arrival: December 31
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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rHuIL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha
Species

Human

Source

E. coli

Accession

P01583

Gene ID

3552

Molecular Weight

Approximately 18.0 kDa

AA Sequence

SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA

Biological Activity
The ED50 is <1.0 pg/mL as measured by murine D10S cells, corresponding to a specific activity of >1.0 × 109 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Interleukin-1 alpha (rHuIL-1α) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA.

Storage & Stability

Lyophilized recombinant Human Interleukin-1 alpha (rHuIL-1α) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Recombinant Human Interleukin-1 alpha is a non-glycosylated, polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton. The purified human monocyte derived IL-1 (mIL-1), a mixture of the two IL-1 forms but predominantly consisting of IL-1 beta, modulates eosinophil oxidative metabolism and enzyme secretion. Although the two major species of IL-1 (IL-1 alpha and IL-1 beta) have identical specific activities on T cells, the selective effects of human recombinant IL-1 (hrIL-1) alpha and hrIL-1 beta are reported on eosinophil function. Recombinant Human Interleukin-1 beta (hrIL-1 beta) causes a significant increase in arylsulfatase secretion (235.4±29% of resting secretion, P≤0.01) and beta-glucuronidase secretion (135.8±9.6% of resting secretion, P≤0.02) similar to mIL-1, Recombinant Human Interleukin-1 alpha (hrIL-1 alpha) has no effect on enzyme secretion. A mixture of hrIL-1 alpha and hrIL-1 beta reproduces the ability of mIL-1 to inhibit the oxidative response to suboptimal doses of phorbol myristate acetate (PMA). When eosinophils are separated into subpopulations by density gradients, eosinophil responses to IL-1 differ among the populations[1]. Members of the IL-1 family such as IL-1α and IL-1β are pro-inflammatory cytokines. Increased IL-1α and IL-1β levels have been detected separately in cigarette smoke (CS)-treated animals and inhibition of the respective signaling pathways result in anti-inflammatory activity[2].

Documentation
References
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Product Name:
Recombinant Human Interleukin-1 alpha
Cat. No.:
HY-P7027
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