1. Recombinant Proteins
  2. Recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed)

Recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed)

Cat. No.: HY-P7377
Handling Instructions

Recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed) is a type I transmembrane glycoprotein that forms a complex with type I transmembrane signal transducer protein gp130 (CD130) and mediates the biological activities of IL-6.

For research use only. We do not sell to patients.

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Recombinant Proteins-MedChemExpress
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50 μg $190 Ask For Quote & Lead Time

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  • Technical Parameters

  • Properties

  • Description

Synonyms
rHuIL-6 Receptor α; gp80; CD126
Species

Human

Source

Sf9 insect cells

Accession

P08887

Gene ID

3570

Molecular Weight

Approximately 50 kDa

AA Sequence

HHHHHHDDDD KLAPRRCPAQ EVARGVLTSL PGDSVTLTCP GVEPEDNATV HWVLRKPAAG SHPSRWAGMG RRLLLRSVQL HDSGNYSCYR AGRPAGTVHL LVDVPPEEPQ LSCFRKSPLS NVVCEWGPRS TPSLTTKAVL LVRKFQNSPA EDFQEPCQYS QESQKFSCQL AVPEGDSSFY IVSMCVASSV GSKFSKTQTF QGCGILQPDP PANITVTAVA RNPRWLSVTW QDPHSWNSSF YRLRFELRYR AERSKTFTTW MVKDLQHHCV IHDAWSGLRH VVQLRAQEEF GQGEWSEWSP EAMGTPWTES RSPPAENEVS TPMQALTTNK DDDNILFRDS ANATSLPVQD

Biological Activity
The ED50 is <50 ng/mL as measured by M1 cells, corresponding to a specific activity of >2 × 104 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed) (rHuIL-6 Receptor α) to 100 µg/mL using ddH2O.

Storage & Stability

Lyophilized recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed) (rHuIL-6 Receptor α) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

It is found that soluble IL-6 receptor alpha-subunit (IL-6Ralpha), with their constitutive IL-6 synthesis, stimulated endothelial cells to synthesize E-selectin, intracellular adhesion molecule-1, vascular cellular adhesion molecule-1, IL-6, and IL-8, and to bind neutrophils. Neutrophils express significant amounts of IL-6Ralpha and upon stimulation shed it: this material activates endothelial cells through a newly constituted IL-6 receptor. Retrograde signaling from PMN activated in the extravascular compartment to surrounding endothelial cells will recruit more and a wider variety of leukocytes. The limiting signal is a soluble receptor, not a cytokine[1].

Documentation
References
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Product Name:
Recombinant Human Interleukin-6 Receptor alpha (Sf9 insect cells-expressed)
Cat. No.:
HY-P7377
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