1. Recombinant Proteins
  2. Recombinant Human MIP-1 alpha/CCL3

Recombinant Human MIP-1 alpha/CCL3

Cat. No.: HY-P7256
Handling Instructions

Recombinant Human MIP-1 alpha/CCL3 is a member of CC subfamily, exerting various biological effects by binding to its three cell surface receptors, including CCR1, CCR3, and CCR5.

For research use only. We do not sell to patients.

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50 μg $420 Ask For Quote & Lead Time

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  • Technical Parameters

  • Properties

  • Description

Synonyms
rHuMIP-1α/CCL3; C-C motif chemokine 3; MIP1A; SCYA3
Species

Human

Source

E. coli

Accession

P10147

Gene ID

6348

Molecular Weight

Approximately 7.8 kDa

AA Sequence

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Biological Activity
Full biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human MIP-1 alpha/CCL3 (rHuMIP-1α/CCL3) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA.

Storage & Stability

Lyophilized recombinant Human MIP-1 alpha/CCL3 (rHuMIP-1α/CCL3) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

CCL3 also known as macrophage inflammatory protein 1-a, is a member of the CC subfamily. It's known that CCL3 is produced by monocytes/macrophages, lymphocytes, neutrophils as well as immune cells such as basophils, mast cells, fibroblasts, and dendritic cells. Meanwhile, CCL3 exerts various biological effects by binding to its three cell surface receptors, including CCR1, CCR3, and CCR5. MIP-1a induces a variety of pro-inflammatory activities such as leukocyte chemotaxis, and promotes the entry of T cells into the inflammatory tissue region from blood circulation. Chemotactic CD4+ cells, CD8+ cells, natural killer cells, and dendritic cells bind to the corresponding receptors and coordinate the occurrence of immune reactions in the immune response site by migrating through vascular endothelial cells. In addition, MIP-1a is considered as a key inflammatory mediator in granuloma, asthma, T1D as well as other autoimmune diseases[1].

Documentation
References
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Recombinant Human MIP-1 alpha/CCL3
Cat. No.:
HY-P7256
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