1. Recombinant Proteins
  2. Recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed)

Recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed)

Cat. No.: HY-P7055A
Handling Instructions

Recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed) is the most active PDGF isoform, binds to PDGF receptor, promotes diverse cell types proliferation and osteogenesis, and further stimulates bone formation in fracture or defect.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $190 In-stock
Estimated Time of Arrival: December 31
50 μg $590 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rHuPDGF-BB; PDGF-2; GDGF; ODGF; SIS; SSV
Species

Human

Source

P. pastoris

Accession

P01127

Gene ID

5155

Molecular Weight

Approximately 24.3 kDa

AA Sequence

SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT

Biological Activity
The ED50 is <3 ng/mL as measured by Balb/c 3T3 cells, corresponding to a specific activity of >3.3 × 105 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 10 mM acetic acid.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed) (rHuPDGF-BB) to 100 µg/mL using ddH2O.

Storage & Stability

Lyophilized recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed) (rHuPDGF-BB) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed) is the most active PDGF isoform, binds to PDGF receptor, promotes diverse cell types proliferation and osteogenesis, and further stimulates bone formation in fracture or defect. Platelet-derived growth factor-BB (PDGF-BB) is primarily secreted from platelet α-granules and is the most active PDGF isoform in bone and other connective tissue as it can bind to all known PDGF receptors. PDGF-BB plays an important role in bone regeneration by inducing mitogenesis, chemotaxis, extracellular matrix formation, and vascularization[1]. Recombinant Human Platelet-derived Growth Factor-BB (rhPDGF-BB) accelerates tendon healing by improving matrix remodeling, increased collagen synthesis, and increased cell proliferation. Recombinant Human Platelet-derived Growth Factor-BB is also efficacious in a non-ruptured, degenerated, tendinopathy model. Furthermore, Recombinant Human Platelet-derived Growth Factor-BB addresses chronic tendinopathies by inducing proliferation and migration of progenitor cells and tenocytes, which stimulate structural repair of the degenerated tendon[2].

Documentation
References
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Product Name:
Recombinant Human Platelet-derived Growth Factor-BB (P.pastoris-expressed)
Cat. No.:
HY-P7055A
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