1. Recombinant Proteins
  2. Recombinant Human Serpin A1/alpha-1-Antitrypsin, His (HEK293-expressed)

Recombinant Human Serpin A1/alpha-1-Antitrypsin, His (HEK293-expressed)

Cat. No.: HY-P7542
Handling Instructions

Recombinant Human Serpin A1/alpha-1-Antitrypsin, His (HEK293-expressed) produced in HEK293 cells is a mouse recombinant Serpin A1a with a His tag at the C-terminus[1]. Alpha-1 Antitrypsin Exerts a hepatoprotective effect on immune-mediated hepatitis and acetaminophen-induced liver injury[2].

For research use only. We do not sell to patients.

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50 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Description

Synonyms
rHuSerpin A1/alpha-1-Antitrypsin, His; Alpha-1-Antitrypsin; Alpha-1 Protease Inhibitor; Alpha-1-Antiproteinase; Serpin A1; SERPINA1; AAT; PI
Species

Human

Source

HEK 293

Accession

P01009

Gene ID

5265

Molecular Weight

50-65 kDa

AA Sequence

EDPQGDAAQK TDTSHHDQDH PTFNKITPNL AEFAFSLYRQ LAHQSNSTNI FFSPVSIATA FAMLSLGTKA DTHDEILEGL NFNLTEIPEA QIHEGFQELL RTLNQPDSQL QLTTGNGLFL SEGLKLVDKF LEDVKKLYHS EAFTVNFGDT EEAKKQINDY VEKGTQGKIV DLVKELDRDT VFALVNYIFF KGKWERPFEV KDTEEEDFHV DQVTTVKVPM MKRLGMFNIQ HCKKLSSWVL LMKYLGNATA IFFLPDEGKL QHLENELTHD IITKFLENED RRSASLHLPK LSITGTYDLK SVLGQLGITK VFSNGADLSG VTEEAPLKLS KAVHKAVLTI DEKGTEAAGA MFLEAIPMSI PPEVKFNKPF VFLMIEQNTK SPLFMGKVVN PTQKHHHHHH

Biological Activity
Data is not available.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Serpin A1/alpha-1-Antitrypsin, His (HEK293-expressed) (rHuSerpin A1/alpha-1-Antitrypsin, His) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability

Lyophilized recombinant Human Serpin A1/alpha-1-Antitrypsin, His (HEK293-expressed) (rHuSerpin A1/alpha-1-Antitrypsin, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Alpha-1-antitrypsin 1-1 (Serpin A1a; AAT) is the most abundant circulating serine protease inhibitor (serpin) and the prototypic member of the serpin super family. The 52-kD Serpin A1a protein is coded by the gene SERPINA1. Serpin A1a plays an important role in modulating immunity, inflammation, proteostasis, apoptosis, and possibly cellular senescence programs. Systemic deficiency in Serpin A1a due to genetic mutations can result in liver failure and chronic lung disease such as emphysema[1].

Documentation
References
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Product Name:
Recombinant Human Serpin A1/alpha-1-Antitrypsin, His (HEK293-expressed)
Cat. No.:
HY-P7542
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