1. Recombinant Proteins
  2. Recombinant Human Signal regulatory protein alpha, His (HEK293-expressed)

Recombinant Human Signal regulatory protein alpha, His (HEK293-expressed)

Cat. No.: HY-P7405
Handling Instructions

Recombinant Human Signal regulatory protein alpha, His (HEK293-expressed) produced in HEK293 cells is a polypeptide chain amino acids with the C-termimal His tag. Signal regulatory protein alpha (SIRPα, designated CD172a) is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily.

For research use only. We do not sell to patients.

Recombinant Proteins

Recombinant Proteins-MedChemExpress
Size Price Stock
10 μg $190 Ask For Quote & Lead Time
50 μg $390 Ask For Quote & Lead Time

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  • Technical Parameters

  • Properties

  • Description

Synonyms
rHuSignal regulatory protein α, His; CD172a; SHPS-1; BIT; MYD1
Species

Human

Source

HEK 293

Accession

P78324

Gene ID

140885

Molecular Weight

50-55 kDa

AA Sequence

EEELQVIQPD KSVLVAAGET ATLRCTATSL IPVGPIQWFR GAGPGRELIY NQKEGHFPRV TTVSDLTKRN NMDFSIRIGN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA PVVSGPAARA TPQHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP VGESVSYSIH STAKVVLTRE DVHSQVICEV AHVTLQGDPL RGTANLSETI RVPPTLEVTQ QPVRAENQVN VTCQVRKFYP QRLQLTWLEN GNVSRTETAS TVTENKDGTY NWMSWLLVNV SAHRDDVKLT CQVEHDGQPA VSKSHDLKVS AHPKEQGSNT AAENTGSNER HHHHHH

Biological Activity
2 µg/mL (100 µL/well) of immoblized recombinant human Signal regulatory protein alpha-His can bind human CD47-Fc with a linear range of 0.25-185 ng/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Signal regulatory protein alpha, His (HEK293-expressed) (rHuSignal regulatory protein α, His) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability

Lyophilized recombinant Human Signal regulatory protein alpha, His (HEK293-expressed) (rHuSignal regulatory protein α, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Signal regulatory protein α (SIRPα) is a glycoprotein receptor that recruits and signals via the tyrosine phosphatases SHP-1 and SHP-2.The cytoplasmic domain of SIRPα contains four immunoreceptor tyrosine-based inhibition motifs (ITIMs), which upon phosphorylation recruit and activate SH2-domain-containing phosphotyrosine phosphatases (PTPase) SHP-1 and SHP-2. In macrophages SIRPα can negatively regulate the phagocytosis of host cells and the production of tumor necrosis factor alpha. SIRPα ligation induces macrophage NO production through the cooperative action of JAK/STAT and PI3-K/Rac1/NOX/H2O2 signaling pathways. It proposes that SIRPα may function as an inhibitory receptor[1].

Documentation
References
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Product Name:
Recombinant Human Signal regulatory protein alpha, His (HEK293-expressed)
Cat. No.:
HY-P7405
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