1. Recombinant Proteins
  2. Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed)

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed)

Cat. No.: HY-P7058A
Handling Instructions

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed) is a strong pro-inflammatory cytokine, exhibits high efficacy and tumor cell targeting capability, with low toxicity effects on healthy cells.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $190 In-stock
Estimated Time of Arrival: December 31
50 μg $240 In-stock
Estimated Time of Arrival: December 31
100 μg $340 In-stock
Estimated Time of Arrival: December 31
250 μg   Get quote  

* Please select Quantity before adding items.

  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rHuTNF-α/TNFSF2; TNF-alpha; Cachectin; DIF; TNFA; Differentiation-inducing factor
Species

Human

Source

P. pastoris

Accession

P01375

Gene ID

7124

Molecular Weight

Approximately 17.4 kDa

AA Sequence

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Biological Activity
The ED50 is <0.08 ng/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >1.25 × 107 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed) (rHuTNF-α/TNFSF2) to 100 µg/mL using ddH2O.

Storage & Stability

Lyophilized recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed) (rHuTNF-α/TNFSF2) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Tumor necrosis factor α (TNF-α, also known as cachectin) is a strong pro-inflammatory cytokine playing an important role in the immune system during inflammation, cell proliferation, differentiation and apoptosis. TNF-α is type II transmembrane protein that can be expressed in both membrane-bound and secreted forms. Receptors for TNF-a are part of TNF receptor (TNFR) superfamily, with characteristic intracellular segment, a transmembrane domain, and an extracellular ligand-binding domain. They are type I transmembrane glycoproteins characterized by a cysteinerich motif (up to 40 aminoacids) repeated 2-6 times in the extracellular N-terminal domain[1]. Tumor necrosis factor-α (TNF-α) is a pleiotropic factor exhibiting antitumor effects, alters endothelial barrier function, reduces tumor interstitial pressure and mediates immune responses. TNF-α is associated with a number of cell signalling events, associated with cell necrosis and apoptosis, and is important for resistance to infection and cancer[2]

Documentation
References
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Product Name:
Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 (P.pastoris-expressed)
Cat. No.:
HY-P7058A
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