1. Recombinant Proteins
  2. Recombinant Mouse Basic Fibroblast Growth Factor

Recombinant Mouse Basic Fibroblast Growth Factor

Cat. No.: HY-P7066
Handling Instructions

Recombinant Mouse Basic Fibroblast Growth Factor is a potent mitogen and chemoattractant for endothelial cells as well as fibroblasts.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $90 In-stock
Estimated Time of Arrival: December 31
50 μg $190 In-stock
Estimated Time of Arrival: December 31
100 μg $290 In-stock
Estimated Time of Arrival: December 31
250 μg   Get quote  

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Publications Citing Use of MCE Recombinant Mouse Basic Fibroblast Growth Factor

  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rMubFGF; HBGF-2; FGF-2; FGF-b; FGF-basic
Species

Mouse

Source

E. coli

Accession

P15655

Gene ID

14173

Molecular Weight

Approximately 16.5 kDa

AA Sequence

MPALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Biological Activity
The ED50 is <1.0 ng/mL as measured by murine balb/c 3T3 cells, corresponding to a specific activity of >1.0 × 106 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Murine Basic Fibroblast Growth Factor (rMubFGF) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA.

Storage & Stability

Lyophilized recombinant Murine Basic Fibroblast Growth Factor (rMubFGF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Basic fibroblast growth factor (bFGF) is a heparin-binding mitogen for mesoderm-derived cells, also acts as a mitogen, differentiation inducing and maintenance factor for many neuroectodermal cells including glial cells, neurons, paraneurons, and their tumor counterparts. The molecule is expressed in several types of neuroectodermal cells. bFGF occurs in many neuronal target tissues, and can prevent ontogenetic as well as lesion-induced neuron death[1]. In vitro, basic fibroblast growth factor induces an angiogenic phenotype in endothelial cells, which includes chemotaxis, mitogenesis, protease production, beta-integrin expression, and tube formation in three-dimensional gels. It acts by binding to specific tyrosine kinase receptors and to cell-associated heparan sulfate proteoglycans[2].

Documentation
References
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Recombinant Mouse Basic Fibroblast Growth Factor
Cat. No.:
HY-P7066
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