1. Recombinant Proteins
  2. Recombinant Mouse CD112, His (HEK293-expressed)

Recombinant Mouse CD112, His (HEK293-expressed)

Cat. No.: HY-P7781
Handling Instructions

Recombinant Mouse CD112, His (HEK293-expressed) is a recombinant mouse CD112 expressed in HEK 293 cells with a His tag at the N-terminus. Recombinant Mouse CD112 can be used as cell surface ligands for the DNAM-1 (CD226) activating molecule[1][2].

For research use only. We do not sell to patients.

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rMuCD112, His; Poliovirus Receptor-Related Protein 2; Herpes Virus Entry Mediator B; Herpesvirus Entry Mediator B; CD112; PVRL2; HVEB; PRR2
Species

Mouse

Source

HEK 293

Accession

P32507

Gene ID

19294

Molecular Weight

40-45 kDa

AA Sequence

QDVRVRVLPEVRGRLGGTVELPCHLLPPTTERVSQVTWQRLDGTVVAAFHPSFGVDFPNSQFSKDRLSFVRARPETNADLRDATLAFRGLRVEDEGNYTCEFATFPNGTRRGVTWLRVIAQPENHAEAQEVTIGPQSVAVARCVSTGGRPPARITWISSLGGEAKDTQEPGIQAGTVTIISRYSLVPVGRADGVKVTCRVEHESFEEPILLPVTLSVRYPPEVSISGYDDNWYLGRSEAILTCDVRSNPEPTDYDWSTTSGVFPASAVAQGSQLLVHSVDRMVNTTFICTATNAVGTGRAEQVILVRESPSTAGAGATGGHHHHHH

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity
Data is not available.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Mouse CD112, His (HEK293-expressed) (rMuCD112, His) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability

Lyophilized recombinant Mouse CD112, His (HEK293-expressed) (rMuCD112, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Nectin-2 (CD112) is a 60 (Nectin-2 α) or 65 kDa (Nectin-2 δ) type I TM glycoprotein that expressed in a variety of cells. Nectin is a Ca2+-independent immunoglobulin-like intercellular adhesion molecule. Nectin comprises a family of at least four members nectin-1, nectin-2, nectin-3 and nectin-4. All the members have two or three splice variants (except nectin-4), and have an extracellular region containing three Ig-like domains, a single transmembrane region and a cytoplasmic region (except nectin-1γ). Nectin-1, nectin-2 and nectin-3 are ubiquitously expressed in a variety of cells. Nectin-2 and nectin-3 are also expressed in cells that lack cadherins. Nectin-4 is expressed mainly in the placenta[1][2].

Documentation
References
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Product Name:
Recombinant Mouse CD112, His (HEK293-expressed)
Cat. No.:
HY-P7781
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