1. Recombinant Proteins
  2. Recombinant Rat Interleukin-1 alpha (CHO-expressed)

Recombinant Rat Interleukin-1 alpha (CHO-expressed)

Cat. No.: HY-P7096
Handling Instructions

Recombinant Rat Interleukin-1 alpha (CHO-expressed) is a nonsecreted pro-inflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells and endothelial cells.

For research use only. We do not sell to patients.

Recombinant Proteins

Recombinant Proteins-MedChemExpress
Size Price Stock
10 μg $190 Ask For Quote & Lead Time
50 μg $490 Ask For Quote & Lead Time
100 μg $780 Ask For Quote & Lead Time

* Please select Quantity before adding items.

  • Technical Parameters

  • Properties

  • Description

Synonyms
rRtIL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha
Species

Rat

Source

CHO

Accession

P16598

Gene ID

24493

Molecular Weight

17-22 kDa

AA Sequence

APHSFQNNLR YKLIRIVKQE FIMNDSLNQN IYVDMDRIHL KAASLNDLQL EVKFDMYAYS SGGDDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS

Biological Activity
The ED50 is <5 pg/mL as measured by D10S cells.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Rat Interleukin-1 alpha (CHO-expressed) (rRtIL-1α) to 100 µg/mL using ddH2O or diluted with PBS.

Storage & Stability

Lyophilized recombinant Rat Interleukin-1 alpha (CHO-expressed) (rRtIL-1α) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

IL-1alpha and IL-1beta have potent effects on the central nervous system resulting in fever, activation of the hypothalamic-pituitary-adrenal axis and behavioural depression. IL-1alpha and IL-1beta show some species specificity in the potency of their biological activities, the objective of the present work was to directly compare the effects of recombinant rat IL-1alpha and IL-1beta in the rat system as a first step to dissect out the mechanisms that are involved in these effects. In vitro, recombinant rat IL-1alpha and IL-1beta bind with the same affinity as human IL-1 to the rat insulinoma Rin m5F cell line that mainly expresses type I IL-1 receptors. This binding activated IL-1 receptors, as shown by induction of the synthesis of TNF-alpha mRNA. In vivo, recombinant rat IL-1alpha and IL-1beta enhance body temperature, increase plasma levels of corticosterone and ACTH, and depress social behavior[1].

Documentation
References
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Recombinant Rat Interleukin-1 alpha (CHO-expressed)
Cat. No.:
HY-P7096
Quantity:
MCE Japan Authorized Agent: