1. Recombinant Proteins
  2. Recombinant Rat Interleukin-5

Recombinant Rat Interleukin-5

Cat. No.: HY-P7102
Handling Instructions

Recombinant Rat Interleukin-5, a T-cell derived cytokine, stimulates B cell growth and increases immunoglobulin secretion.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock
10 μg $190 Ask For Quote & Lead Time
50 μg $620 Ask For Quote & Lead Time
100 μg $1050 Ask For Quote & Lead Time

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  • Technical Parameters

  • Properties

  • Description

Synonyms
rRtIL-5; EDF; BCDFII; TRF
Species

Rat

Source

E. coli

Accession

Q9R2C9

Gene ID

24497

Molecular Weight

Approximately 26.0 kDa

AA Sequence

MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV

Biological Activity
The ED50 is <0.4 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >2.5 × 106 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM Tris, pH 8.5.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

Reconstitute the lyophilized recombinant Rat Interleukin-5 (rRtIL-5) to 100 µg/mL using ddH2O.

Storage & Stability

Lyophilized recombinant Rat Interleukin-5 (rRtIL-5) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Interleukin-5 (IL-5) is a T-cell derived cytokine which stimulates eosinophil production and activation in human, mice and sheep. IL-5 is active as a growth factor for mouse but not human B cells. The role of IL-5 on ruminant B cells has not been clearly defined. By hybridisation with human IL-5 cDNA, the ovine IL-5 gene is isolated from a liver genomic library. The IL-5 cDNA is obtained by reverse-transcriptase PCR using primers designed from the 5' and 3' coding sequence derived from the ovine IL-5 gene. The sequences of the cDNA shows that there is 79% and 73% nucleotide homology with the human and mouse sequences. The ovine IL-5 cDNA encodes a protein of 132 amino acids and the level of amino acid homology with human and mouse IL-5 is 64% and 56%, respectively. Two cysteine residues are conserved in ovine, human and mouse IL-5. There are two potential N-linked glycoyslation sites in ovine IL-5[1].

Documentation
References
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Product Name:
Recombinant Rat Interleukin-5
Cat. No.:
HY-P7102
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