1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a Protein, Human (CAA71403, HEK293, Fc)

SIRP alpha/CD172a Protein, Human (CAA71403, HEK293, Fc)

Cat. No.: HY-P70689
Handling Instructions

SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is a recombinant protein expressed in HEK 293 cells. SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is expressed with the Fc region of mouse IgG2a at the C-terminus.

For research use only. We do not sell to patients.

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Description

SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is a recombinant protein expressed in HEK 293 cells. SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is expressed with the Fc region of mouse IgG2a at the C-terminus.

Background

SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells[1].

Species

Human

Source

HEK293

Tag

C-hFc

Accession

CAA71403.1 (E31-R370)

Gene ID
Molecular Construction
N-term
CD172a (E31-R370)
Accession # CAA71403.1
hFc
C-term
Synonyms
Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; SHPS-1; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP
AA Sequence

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

Molecular Weight

85-105 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SIRP alpha/CD172a Protein, Human (CAA71403, HEK293, Fc)
Cat. No.:
HY-P70689
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