1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. SIRP alpha/CD172a Immunoglobulin-like Cell Adhesion Molecules
  5. Signal Regulatory Protein α
  6. SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc)

SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc)

Cat. No.: HY-P70689
COA Handling Instructions

SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is a recombinant protein expressed in HEK 293 cells. SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is expressed with the Fc region of mouse IgG2a at the C-terminus.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
10 μg $110 In-stock
Estimated Time of Arrival: December 31
50 μg $330 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is a recombinant protein expressed in HEK 293 cells. SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc) is expressed with the Fc region of mouse IgG2a at the C-terminus.

Background

SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells[1].

Species

Human

Source

HEK293

Tag

C-hFc

Accession

CAA71403.1 (E31-R370)

Gene ID

140885  [NCBI]

Synonyms
Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; SHPS-1; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP
AA Sequence

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

Molecular Weight

85-105 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SIRP alpha/CD172a Protein, Human (CAA71403.1, HEK 293, Fc)
Cat. No.:
HY-P70689
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