1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. T Cell CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Pattern Recognition Receptors
  4. CD283/TLR3 Toll-like Receptor
  5. Toll-like Receptor 3
  6. TLR3 Protein, Mouse (sf9, His)

TLR3 Protein, Mouse (sf9, His)

Cat. No.: HY-P73587
Handling Instructions

TLR3 protein is an important component of innate and adaptive immunity and can recognize pathogen-associated molecular patterns and activate NF-κ-B, IRF3 and cytokine secretion. Ligand binding induces TLR3 dimerization, and efficient signal transduction requires clustering of signaling units along the ligand. TLR3 Protein, Mouse (sf9, His) is the recombinant mouse-derived TLR3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of TLR3 Protein, Mouse (sf9, His) is 677 a.a., with molecular weight of 75-80 kDa.

For research use only. We do not sell to patients.

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Description

TLR3 protein is an important component of innate and adaptive immunity and can recognize pathogen-associated molecular patterns and activate NF-κ-B, IRF3 and cytokine secretion. Ligand binding induces TLR3 dimerization, and efficient signal transduction requires clustering of signaling units along the ligand. TLR3 Protein, Mouse (sf9, His) is the recombinant mouse-derived TLR3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of TLR3 Protein, Mouse (sf9, His) is 677 a.a., with molecular weight of 75-80 kDa.

Background

TLR3 protein serves as a key component in both innate and adaptive immunity, playing a pivotal role in the host immune response against pathogens by recognizing specific molecular patterns associated with microorganisms. Activated by double-stranded RNA, a characteristic feature of viral infection, TLR3 functions through the adapter TRIF/TICAM1, leading to NF-kappa-B activation, nuclear translocation of IRF3, cytokine secretion, and initiation of the inflammatory response. TLR3 exists as a monomer and homodimer, with ligand-binding triggering dimerization. The signaling unit comprises one approximately 40 bp ds-RNA and two TLR3 molecules, requiring lateral clustering of signaling units along the ds-RNA ligand for effective signal transduction. Interaction with UNC93B1, SRC, and TICAM1, particularly in response to poly(I:C), contributes to its regulatory functions, highlighting the intricate molecular mechanisms involved in TLR3-mediated immune responses.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His

Accession

Q99MB1 (C29-L705)

Gene ID
Molecular Construction
N-term
His
TLR3 (C29-L705)
Accession # Q99MB1
C-term
Synonyms
Toll-like receptor 3; CD283; Tlr3
Molecular Weight

75-80 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TLR3 Protein, Mouse (sf9, His)
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HY-P73587
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