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  5. TNF-alpha/TNFSF2 Protein, Human (His)

TNF-alpha/TNFSF2 Protein, Human (His)

Cat. No.: HY-P7416
Handling Instructions

TNF-alpha/TNFSF2 Protein, Human (His) is recombinant human tumor necrosis factor alpha (TNF-α) with his tag. TNF-alpha/TNFSF2 Protein, a pleiotropic cytokine, is one of the major mediators of inflammation and is involved in a number of infectious and parasitic diseases. It appears to be involved in blood-brain barrier, inflammatory, thrombogenic, and vascular changes associated with brain injury.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $100 In-stock
Estimated Time of Arrival: December 31
50 μg $220 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

E. coli

Tag

N-His

Accession

P01375 (V77-L233)

Gene ID

7124

Synonyms
rHuTNF-α, His; Cachectin; TNFSF2
AA Sequence

MHHHHHHVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Molecular Weight

Approximately 18.3 kDa

Biological Activity
The ED50 is <0.13 ng/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >7.69 × 106 units/mg.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<1.0 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine produced by a wide variety of cell types of mostly hematopoietic, but also of nonhematopoietic, origin. TNFα is instrumental in the immune elimination of various infectious agents such as Candida albicans, Listeria monocytogenes, or mycobacteria and exerts potent proinflammatory effects, e.g. by inducing the expression of adhesion molecules such as VCAM-1, intercellular adhesion molecule 1 (ICAM-1), or E-selectin on endothelial cells and other cell types. Aberrant production of TNFα, however, has been also implicated in the pathogenesis of various diseases, such as rheumatoid arthritis, insulin-dependent diabetes-mellitus, sialoadenitis, and inflammatory bowel disease, in particular Crohn's disease[1][2].

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
TNF-alpha/TNFSF2 Protein, Human (His)
Cat. No.:
HY-P7416
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MCE Japan Authorized Agent: