AF555

By Targets:	Biochemical Assay Reagents (1) Fluorescent Dye (7) Microtubule/Tubulin (1)
By Research Areas:	Cancer (2)
Click Chemistry:	Azide (1)

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-NP163C

Wheat germ agglutinin-AF555 WGA-AF555	Fluorescent Dye	Others
Wheat germ agglutinin-AF555 (WGA-AF555) is a membrane-staining lectin conjugate that combines wheat germ agglutinin with the Alexa Fluor 555 fluorescent dye. Wheat germ agglutinin-AF555 is used for precise staining and contour delineation of cell membranes. Wheat germ agglutinin-AF555 also effectively distinguishes between surface vimentin and intracellular vimentin in cells .

HY-D2175

AF 555 NHS ester	Fluorescent Dye	Others
AF 555 NHS ester is a bright, orange-red fluorescence probe. AF 555 NHS ester can be used to label primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules (Ex/Em = 552/566 nm) .

HY-D2177

AF 555 azide	Fluorescent Dye	Others
AF 555 azide is a fluorescent dye and is the azide of AF 555. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 555/565 nm) .

HY-D2444

AF555 NHS	Fluorescent Dye	Cancer
AF555 NHS is a red fluorescent dye with excellent fluorescence properties and light stability. The excitation wavelength is 556 nm and the emission wavelength is 571 nm, which can be used for protein labeling, antibody labeling, and cell imaging .

HY-D1710

*Anti-α-Tubulin Antibody, AF555* conjugate**	Microtubule/Tubulin	Others
Anti-α-Tubulin Antibody, AF555 conjugate is a conjugate of mouse anti-α-tubulin monoclonal antibody and the red fluorescent dye Alexa Fluor 555. Anti-α-Tubulin Antibody, AF555 conjugate can be used for the detection of tubulin (Ex/Em: 554/567 nm) .

HY-D2176

AF 555 carboxylic acid	Fluorescent Dye	Others
AF 555 carboxylic acid is a derivative of the orange fluorescent dye AF 555. AF 555 carboxylic acid is widely used in cell dyes, biological dyes, biomolecules and particle fluorescent labeling. AF 555 exhibits average excitation wavelengths under green laser and red laser of 510 nm and 610 nm, respectively .

HY-NP057

Ovalbumin-AF555 OVA-AF555	Biochemical Assay Reagents	Others
Ovalbumin-AF555 is an AF555 conjugated ovalbumin (Ex=752 nM, Em=780 nm). Ovalbumin-AF555 can be used in the research of cell interaction .

HY-NP164J

Concanavalin A-AF555 Con A-AF555	Fluorescent Dye	Cancer
Concanavalin A-AF555 (Con A-AF555) is an AF555-labled Concanavalin A (HY-P2149). Concanavalin A is a Ca ²⁺/Mn ²⁺-dependent and mannose/glucose-binding plant lectin. Concanavalin A binds the N- and O-glycosylated proteins of head and neck carcinoma cells glycoproteins .

HY-W800702

*BP Fluor 555* azide**	Fluorescent Dye	Others
BP Fluor 555 Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. BP Fluor 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. AF 555 conjugates ideally suited for detection of low abundance targets. BP Fluor 555 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Cat. No.	Product Name	Type

HY-D2175

AF 555 NHS ester	Fluorescent Dye
AF 555 NHS ester is a bright, orange-red fluorescence probe. AF 555 NHS ester can be used to label primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules (Ex/Em = 552/566 nm) .

HY-D2177

AF 555 azide	Fluorescent Dye
AF 555 azide is a fluorescent dye and is the azide of AF 555. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 555/565 nm) .

HY-D2444

AF555 NHS	Fluorescent Dye
AF555 NHS is a red fluorescent dye with excellent fluorescence properties and light stability. The excitation wavelength is 556 nm and the emission wavelength is 571 nm, which can be used for protein labeling, antibody labeling, and cell imaging .

HY-D2176

AF 555 carboxylic acid	Fluorescent Dye
AF 555 carboxylic acid is a derivative of the orange fluorescent dye AF 555. AF 555 carboxylic acid is widely used in cell dyes, biological dyes, biomolecules and particle fluorescent labeling. AF 555 exhibits average excitation wavelengths under green laser and red laser of 510 nm and 610 nm, respectively .

HY-W800702

*BP Fluor 555* azide**	Fluorescent Dye
BP Fluor 555 Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. BP Fluor 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. AF 555 conjugates ideally suited for detection of low abundance targets. BP Fluor 555 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

BP Fluor 555 azide

Fluorescent Dye

BP Fluor 555 Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. BP Fluor 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. AF 555 conjugates ideally suited for detection of low abundance targets. BP Fluor 555 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Cat. No.	Product Name	Type

HY-NP163C

Wheat germ agglutinin-AF555 WGA-AF555	Biochemical Assay Reagents
Wheat germ agglutinin-AF555 (WGA-AF555) is a membrane-staining lectin conjugate that combines wheat germ agglutinin with the Alexa Fluor 555 fluorescent dye. Wheat germ agglutinin-AF555 is used for precise staining and contour delineation of cell membranes. Wheat germ agglutinin-AF555 also effectively distinguishes between surface vimentin and intracellular vimentin in cells .

Wheat germ agglutinin-AF555

WGA-AF555

Biochemical Assay Reagents

Wheat germ agglutinin-AF555 (WGA-AF555) is a membrane-staining lectin conjugate that combines wheat germ agglutinin with the Alexa Fluor 555 fluorescent dye. Wheat germ agglutinin-AF555 is used for precise staining and contour delineation of cell membranes. Wheat germ agglutinin-AF555 also effectively distinguishes between surface vimentin and intracellular vimentin in cells .

HY-NP057

Ovalbumin-AF555 OVA-AF555	Biochemical Assay Reagents
Ovalbumin-AF555 is an AF555 conjugated ovalbumin (Ex=752 nM, Em=780 nm). Ovalbumin-AF555 can be used in the research of cell interaction .

HY-NP164J

Concanavalin A-AF555 Con A-AF555	Biochemical Assay Reagents
Concanavalin A-AF555 (Con A-AF555) is an AF555-labled Concanavalin A (HY-P2149). Concanavalin A is a Ca ²⁺/Mn ²⁺-dependent and mannose/glucose-binding plant lectin. Concanavalin A binds the N- and O-glycosylated proteins of head and neck carcinoma cells glycoproteins .

Cat. No.	Product Name

HY-KD1104

*Red Fluorescent Rapid Labeling Kit (AF555）*
Red Fluorescent Rapid Labelling Kit (AF555) enables red fluorescent labelling of proteins. Maximum excitation/ Emission Wavelength: 555/565 nm. Based on NHS ester chemistry, the NHS ester-activated fluorescent dye reacts with primary amines in pH 7-9 solutions to form stable amide bonds with the target antibody/protein, achieving conjugation. Typically, one IgG molecule can bind 2-8 molecules of AF555. The entire procedure can be completed within two hours.

Red Fluorescent Rapid Labeling Kit (AF555）

Red Fluorescent Rapid Labelling Kit (AF555) enables red fluorescent labelling of proteins. Maximum excitation/ Emission Wavelength: 555/565 nm. Based on NHS ester chemistry, the NHS ester-activated fluorescent dye reacts with primary amines in pH 7-9 solutions to form stable amide bonds with the target antibody/protein, achieving conjugation. Typically, one IgG molecule can bind 2-8 molecules of AF555. The entire procedure can be completed within two hours.

Host:	Donkey (2) Rabbit (3) Goat (6)
Reactivity:	Human (1) Mouse (3) Rat (2) Rabbit (2) Goat (2) Chicken (1)
Application:	IHC-P (4) ICC/IF (5) IF-Tissue (7) FC (10) ELISA (10)
Isotype:	IgG (2)
Conjugation:	AF 555 (11)
Clonality:	Polyclonal (11)
Modification:	Unmodified (2)

Cat. No.	Compare	Product Name	Application	Reactivity	Image

HY-P89202

	AF555-conjugated Goat Anti-Rat IgG H&L	IHC-P, ICC/IF, FC, ELISA	Rat
	AF555-conjugated Goat Anti-Rat IgG H&L is an AF555-conjugated, Goat-derived anti-Rat antibody. AF555-conjugated Goat Anti-Rat IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89283

	AF555-conjugated Donkey Anti-Rabbit IgG H&L	ICC/IF, IF-Tissue	Rabbit
	AF555-conjugated Donkey Anti-Rabbit IgG H&L is an AF555-conjugated, Donkey-derived anti-Rabbit antibody. AF555-conjugated Donkey Anti-Rabbit IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89305

	AF555-conjugated Donkey Anti-Goat IgG H&L	IHC-P, ICC/IF, FC, ELISA	Goat
	AF555-conjugated Donkey Anti-Goat IgG H&L is an AF555-conjugated, Donkey-derived anti-Goat antibody. AF555-conjugated Donkey Anti-Goat IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89224

	AF555-conjugated Rabbit Anti-Rat IgG H&L	IF-Tissue, FC, ELISA	Rat
	AF555-conjugated Rabbit Anti-Rat IgG H&L is an AF555-conjugated, Rabbit-derived anti-Rat antibody. AF555-conjugated Rabbit Anti-Rat IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89243

	AF555-conjugated Goat Anti-Human IgG H&L	IF-Tissue, FC, ELISA	Human
	AF555-conjugated Goat Anti-Human IgG H&L is an AF555-conjugated, Goat-derived anti-Human antibody. AF555-conjugated Goat Anti-Human IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89264

	AF555-conjugated Goat Anti-Chicken IgY H&L	IF-Tissue, FC, ELISA	Chicken
	AF555-conjugated Goat Anti-Chicken IgY H&L is an AF555-conjugated, Goat-derived anti-Chicken antibody. AF555-conjugated Goat Anti-Chicken IgY H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89326

	AF555-conjugated Rabbit Anti-Goat IgG H&L	IHC-P, ICC/IF, FC, ELISA	Goat
	AF555-conjugated Rabbit Anti-Goat IgG H&L is an AF555-conjugated, Rabbit-derived anti-Goat antibody. AF555-conjugated Rabbit Anti-Goat IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89346

	AF555-conjugated Rabbit Anti-Mouse IgG H&L	IF-Tissue, FC, ELISA	Mouse
	AF555-conjugated Rabbit Anti-Mouse IgG H&L is an AF555-conjugated, Rabbit-derived anti-Mouse antibody. AF555-conjugated Rabbit Anti-Mouse IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

HY-P89389

	AF555-conjugated Goat Anti-Mouse IgM Mu Heavy Chain	IF-Tissue, FC, ELISA	Mouse
	AF555-conjugated Goat Anti-Mouse IgM Mu Heavy Chain is an AF555-conjugated, Goat-derived anti-Mouse antibody. AF555-conjugated Goat Anti-Mouse IgM Mu Heavy Chain conjugates mouse IgM Mu Heavy Chain.

HY-P81005

	AF555-conjugated Goat Anti-Rabbit IgG H&L 1 Publications Verification Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555); Immunoglobulin G;	FC, ELISA, IF-Tissue	Rabbit
	AF555-conjugated Goat Anti-Rabbit IgG H&Lis an -conjugated, goat-derived anti-rabbit IgG antibody. AF555-conjugated Goat Anti-Rabbit IgG H&L conjugates the light and heavy chains of rabbit IgG antibodies.

HY-P81006

	AF555-conjugated Goat Anti-Mouse IgG H&L Goat Anti-Mouse IgG H&L (Alexa Fluor® 555); Immunoglobulin G;	IHC-P, ICC/IF, FC, ELISA	Mouse
	AF555-conjugated Goat Anti-Mouse IgG H&Lis an -conjugated, goat-derived anti-mouse IgG antibody. AF555-conjugated Goat Anti-Mouse IgG H&L conjugates the light and heavy chains of mouse IgG antibodies.

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Cat. No.	Product Name		Classification

HY-D2177

AF 555 azide		Azide
AF 555 azide is a fluorescent dye and is the azide of AF 555. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups (Ex/Em = 555/565 nm) .

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Remarks

Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

MCE Kits

Antibodies

Click Chemistry