lysate

By Targets:	Acyltransferase (2) AMPK (1) Antibiotic (1) Apoptosis (2) Autophagy (1) Btk (1) CaMK (1) Caspase (1) Cathepsin (1) Endogenous Metabolite (1) Epigenetic Reader Domain (1) Fluorescent Dye (5) HDAC (1) Ketohexokinase (2) Parasite (1) PARP (1) Syk (2) Tyrosinase (1)
By Research Areas:	Cancer (9) Infection (6) Inflammation/Immunology (4) Metabolic Disease (5)
Click Chemistry:	Alkynes (2)

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-B0496

PMSF Maximum Cited Publications 64 Publications Verification Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride	Cathepsin	Others
PMSF is an irreversible serine/cysteine protease inhibitor commonly used in the preparation of cell lysates.

HY-100894

Biotin-VAD-FMK

Caspase Cancer

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

Biotin-VAD-FMK	Caspase	Cancer
Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

HY-12425

DGAT1-IN-1 1 Publications Verification	Acyltransferase	Metabolic Disease
DGAT1-IN-1 is a potent DGAT1 inhibitor with IC50 of < 10 nM(cell lysate from Hep3B cells overexpressing human DGAT1).

HY-152194

Tyrosinase-IN-10	Tyrosinase	Cancer
Tyrosinase-IN-10 (Compound 23) is a partially competitive tyrosinase inhibitor with an IC₅₀ of 1.6 μM against tyrosinase activity from human melanoma cell lysates .

HY-P10142

Ac-YEVD-AMC	Fluorescent Dye	Others
Ac-YEVD-AMC is a fluorescent substrate of caspase-1/11. Ac-YEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection when treating with cell lysate .

HY-N10197

Pulixin	Parasite Endogenous Metabolite	Infection
Pulixin prevents FREP1 from binding to P. falciparum-infected cell lysate. Pulixin blocks the transmission of the parasite to mosquitoes with an EC₅₀ of 11 µM. Pulixin also inhibits the proliferation of asexual-stage P. falciparum with an EC₅₀ of 47 nM .

HY-126943

SAHA-BPyne	HDAC	Others
SAHA-BPyne is an activity-based protein profiling (ABPP) probe for detecting HDAC activity, which covalently labels the proximal proteins through a photoactivation. SAHA-BPyne inhibits HDAC activity in HeLa nuclear lysate with an IC₅₀ of less than 5 μM .

HY-110013

Terreic acid	Btk Antibiotic	Infection Inflammation/Immunology
Terreic acid, a quinone epoxide antibiotic, acts as an effective Btk inhibitor. Terreic acid blocks the interaction between PKC and the pleckstrin homology domain of Btk. Terreic acid inhibits the binding of GST-BtkPH to PKC in lysates of HMC-1 human mast cells with an IC₅₀ of approximately 100 μM .

HY-P1003

Ac-DEVD-AMC 4 Publications Verification	Fluorescent Dye	Others
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .

HY-110381

JNJ-DGAT2-A	Acyltransferase	Metabolic Disease
JNJ-DGAT2-A is a selective diacylglycerol acyltransferase 2 (DGAT2) inhibitor with an IC₅₀ value of 0.14 μM in human DGAT2-expressing Sf9 insect cell membranes. JNJ-DGAT2-A can be used for the research of triglyceride (TG) synthesis .

HY-D2041

3-HTC	Fluorescent Dye	Cancer
3-HTC is a chemical fluorescent probe. 3-HTC reacts reversibly with thiols and disulfides, and can be used to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates (λ_max: 448 nm in its reduced thiolate form, and a λ_max 370-410 nm for the oxidized mixed disulfide) .

HY-12841A

KHK-IN-1 hydrochloride	Ketohexokinase	Metabolic Disease
KHK-IN-1 hydrochloride (compound 8) is a selective and cell membrane permeable ketohexokinase (KHK) inhibitor (IC₅₀=12 nM; F=34%). KHK-IN-1 hydrochloride inhibits the production of F1P in HepG2 cell lysates (IC>sub>50=400 nM). KHK-IN-1 hydrochloride has potential for the study of diabetes and obesity .

HY-12841

KHK-IN-1	Ketohexokinase	Metabolic Disease
KHK-IN-1 (compound 8) is a selective and cell membrane permeable ketohexokinase (KHK) inhibitor (IC₅₀=12 nM; F=34%). KHK-IN-1 inhibits the production of F1P in HepG2 cell lysates (IC>sub>50=400 nM). KHK-IN-1 has potential for the study of diabetes and obesity .

HY-19805

STO-609 15+ Cited Publications	CaMK AMPK Autophagy	Metabolic Disease
STO-609 is a selective and cell-permeable inhibitor of the Ca ²⁺/calmodulin-dependent protein kinase kinase (CaM-KK), with K_i values of 80 and 15 ng/mL for recombinant CaM-KKα and CaM-KKβ, respectively. STO-609 inhibits AMP-activated protein kinase kinase (AMPKK) activity in HeLa cell lysates with an IC₅₀ ~0.02 g/ml.

HY-19805A

STO-609 acetate
STO-609 acetate is a selective and cell-permeable inhibitor of the Ca ²⁺/calmodulin-dependent protein kinase kinase (CaM-KK), with K_i values of 80 and 15 ng/mL for recombinant CaM-KKα and CaM-KKβ, respectively. STO-609 acetate inhibits AMP-activated protein kinase kinase (AMPKK) activity in HeLa cell lysates with an IC₅₀ ~0.02 g/ml.

HY-D1261

Naph-EA-mal 1 Publications Verification Thiol-green 1	Fluorescent Dye	Others
Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells . Ex: 488 nM; Em: 540 nM.

HY-142684

Pyruvate Carboxylase-IN-1	Others	Cancer
Pyruvate Carboxylase-IN-1 (compound 37) is a potent inhibitor of pyruvate carboxylase (PC) with IC₅₀s of 0.204 and 0.104 μM in cell lysate-based and cell-based PC activity, respectively. Pyruvate Carboxylase-IN-1 is a natural analog of erianin. Pyruvate Carboxylase-IN-1 inhibites the enzymatic activity of PC, mediating the anticancer effect in human hepatocellular carcinoma (HCC) .

HY-142685

Pyruvate Carboxylase-IN-2	Others	Cancer
Pyruvate Carboxylase-IN-2 (compound 29) is a potent inhibitor of pyruvate carboxylase (PC) with IC₅₀s of 0.065 and 0.097 μM in cell lysate-based and cell-based PC activity, respectively. Pyruvate Carboxylase-IN-2 is a natural analog of erianin. Pyruvate Carboxylase-IN-2 inhibites the enzymatic activity of PC, mediating the anticancer effect in human hepatocellular carcinoma (HCC) .

HY-76474

BAY 61-3606	Syk Apoptosis	Inflammation/Immunology Cancer
BAY 61-3606 is an orally available, ATP-competitive, reversible and highly selective Syk inhibitor with a K_i of 7.5 nM and an IC₅₀ of 10 nM . BAY 61-3606 reduces ERK1/2 and Akt phosphorylation in neuroblastoma cell . BAY 61-3606 induces a large decrease of Syk phosphorylation in K-rn cell lysates . Bay 61-3606 sensitizes TRAIL-induced apoptosis by downregulating Mcl-1 in breast cancer cells .

HY-14985

BAY 61-3606 dihydrochloride 5 Publications Verification	Syk Apoptosis	Inflammation/Immunology Cancer
BAY 61-3606 dihydrochloride is an orally available, ATP-competitive, reversible and highly selective Syk inhibitor with a K_i of 7.5 nM an IC₅₀ of 10 nM . BAY 61-3606 dihydrochloride reduces ERK1/2 and Akt phosphorylation in neuroblastoma cell . BAY 61-3606 dihydrochloride induces a large decrease of Syk phosphorylation in K-rn cell lysates . Bay 61-3606 dihydrochloride sensitizes TRAIL-induced apoptosis by downregulating Mcl-1 in breast cancer cells .

HY-143285

AMC-GlcNAc	Fluorescent Dye
AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .

HY-145749

PARPYnD	PARP	Cancer
PARPYnD is a PARP enzyme photoaffinity probe (AfBP) based on the triple PARP1/2/6 inhibitor AZ9482 (HY-119653), which induces breast cancer Formation of multipolar spindles (MPS) in cells. PARPYnD inhibits PAPR wih IC₅₀ of 38 nM (PARP1), 6 nM (PARP2), 230 nM (PARP6), respectively. PARPYnD enriches recombinant PARP6 incorporated into cell lysates and inhibits PARP6 in cell-free assays, but it does not label PARP6 in intact cells .

HY-151532

PBRM1-BD2-IN-5	Epigenetic Reader Domain	Cancer
PBRM1-BD2-IN-5 is a potent PBRM1 Bromodomain inhibitor with K_d values of 1.5 μM and 3.9 μM for PBRM1-BD2 and PBRM1-BD5, respectively, and an IC₅₀ value of 0.26 μM for PBRM1-BD2. PBRM1-BD2-IN-5 reduces the binding of full-length PBRM1 within the PBAF complex in cell lysates to acetylated histone peptide. PBRM1-BD2-IN-5 can be used to research anticancer .

Cat. No.	Product Name	Type

HY-P1003

Ac-DEVD-AMC 4 Publications Verification	Chromogenic Substrates
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .

HY-D1261

Naph-EA-mal 1 Publications Verification Thiol-green 1	Fluorescent Dyes/Probes
Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells . Ex: 488 nM; Em: 540 nM.

HY-D2041

3-HTC	Fluorescent Dyes/Probes
3-HTC is a chemical fluorescent probe. 3-HTC reacts reversibly with thiols and disulfides, and can be used to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates (λ_max: 448 nm in its reduced thiolate form, and a λ_max 370-410 nm for the oxidized mixed disulfide) .

HY-143285

AMC-GlcNAc	Fluorescent Dyes/Probes
AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .

AMC-GlcNAc

Fluorescent Dyes/Probes

AMC-GlcNAc (compound 1) is a potent fluorogenic probe for detection and characterization of β-hexosaminidase enzyme activity. AMC-GlcNAc enables continuous monitoring by turn-on fluorescence and fluorescence signal is constant over a wide pH range (Ex=325 nm, Em=390 nm). AMC-GlcNAc enables ratiometric fluorescence detection with high sensitivity and low background, which can be used to screen for recombinant dispersin B activity in E. coli cell lysate .

Cat. No.	Product Name	Type

HY-100894

Biotin-VAD-FMK

Cell Assay Reagents

Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

Biotin-VAD-FMK	Cell Assay Reagents
Biotin-VAD-FMK is a cell permeable, irreversible biotin-labeled caspase inhibitor, used to identify active caspases in cell lysates.

Cat. No.	Product Name	Target	Research Area

HY-P5650

Bombolitin I

Peptides Infection

Bombolitin I is an antimicrobial peptide derived from bumblebee venom. Bombolitin I can lysate erythrocyte and liposome .
HY-P5652

Bombolitin II

Peptides Infection

Bombolitin II is an antimicrobial peptide derived from bumblebee venom. Bombolitin II can lysate erythrocyte and liposome .
HY-P5653

Bombolitin III

Peptides Infection

Bombolitin III is an antimicrobial peptide derived from bumblebee venom. Bombolitin III can lysate erythrocyte and liposome .
HY-P5654

Bombolitin IV

Peptides Infection

Bombolitin IV is an antimicrobial peptide derived from bumblebee venom. Bombolitin IV can lysate erythrocyte and liposome .

Bombolitin I	Peptides	Infection
Bombolitin I is an antimicrobial peptide derived from bumblebee venom. Bombolitin I can lysate erythrocyte and liposome .

Bombolitin II	Peptides	Infection
Bombolitin II is an antimicrobial peptide derived from bumblebee venom. Bombolitin II can lysate erythrocyte and liposome .

Bombolitin III	Peptides	Infection
Bombolitin III is an antimicrobial peptide derived from bumblebee venom. Bombolitin III can lysate erythrocyte and liposome .

Bombolitin IV	Peptides	Infection
Bombolitin IV is an antimicrobial peptide derived from bumblebee venom. Bombolitin IV can lysate erythrocyte and liposome .

HY-P10142

Ac-YEVD-AMC	Fluorescent Dye	Others
Ac-YEVD-AMC is a fluorescent substrate of caspase-1/11. Ac-YEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection when treating with cell lysate .

HY-106279

EA-230	Peptides	Inflammation/Immunology
EA-230 is a synthetic oligopeptide originally derived from beta-human chorionic gonadotropin (beta-hCG) lysates. EA-230 has anti-inflammatory effects and can be used for the research of sepsis .

HY-P1003

Ac-DEVD-AMC 4 Publications Verification	Fluorescent Dye	Others
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .

HY-P5377

Abz-HPGGPQ-EDDnp Cathepsin K substrate	Peptides	Others
Abz-HPGGPQ-EDDnp (Cathepsin K substrate) is a biological active peptide. (Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.This FRET peptide can be used to monitor selectively cathepsin K activity in physiological fluids and cell lysates. Abz-HPGGPQ-EDDnp [where Abz represents o-aminobenzoic acid and EDDnp represents N -(2, 4-dinitrophenyl)-ethylenediamine], a substrate initially developed for trypanosomal enzymes, is efficiently cleaved at the Gly-Gly bond by cathepsin K. This peptide is resistant to hydrolysis by cathepsins B, F, H, L, S and V, Ex/Em=340 nm/420 nm.)

Cat. No.	Product Name

HY-K0010

Protease Inhibitor Cocktail

975+ Cited Publications

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.
HY-K0011

Protease Inhibitor Cocktail, mini-Tablet

185+ Cited Publications

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Protease Inhibitor Cocktail 975+ Cited Publications
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Protease Inhibitor Cocktail, mini-Tablet 185+ Cited Publications
Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Cat. No.	Product Name	Category	Target	Chemical Structure

HY-N10197

Pulixin	Infection Alkaloids Structural Classification Microorganisms Classification of Application Fields Other Alkaloids Source classification Coumarins Phenols Polyphenols Phenylpropanoids Disease Research Fields	Parasite Endogenous Metabolite
Pulixin prevents FREP1 from binding to P. falciparum-infected cell lysate. Pulixin blocks the transmission of the parasite to mosquitoes with an EC₅₀ of 11 µM. Pulixin also inhibits the proliferation of asexual-stage P. falciparum with an EC₅₀ of 47 nM .

Cat. No.	Product Name		Classification

HY-126943

SAHA-BPyne		Alkynes
SAHA-BPyne is an activity-based protein profiling (ABPP) probe for detecting HDAC activity, which covalently labels the proximal proteins through a photoactivation. SAHA-BPyne inhibits HDAC activity in HeLa nuclear lysate with an IC₅₀ of less than 5 μM .

HY-145749

PARPYnD		Alkynes
PARPYnD is a PARP enzyme photoaffinity probe (AfBP) based on the triple PARP1/2/6 inhibitor AZ9482 (HY-119653), which induces breast cancer Formation of multipolar spindles (MPS) in cells. PARPYnD inhibits PAPR wih IC₅₀ of 38 nM (PARP1), 6 nM (PARP2), 230 nM (PARP6), respectively. PARPYnD enriches recombinant PARP6 incorporated into cell lysates and inhibits PARP6 in cell-free assays, but it does not label PARP6 in intact cells .

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