Tipepidine hydrochloride

Name: Tipepidine hydrochloride
Brand: MedChemExpress
SKU: HY-121685A
Rating: 4.5 (1 reviews)

Cat. No.: HY-121685A Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tipepidine hydrochloride reversibly inhibits dopamine (DA) D₂ receptor-mediated GIRK currents (I_DA(GIRK)) with an IC₅₀ of 7.0 μM. Tipepidine hydrochloride subsequently activates VTA dopamine neuron. Tipepidine hydrochloride, a non-narcotic antitussive, exerts an antidepressant-like effect.

For research use only. We do not sell to patients.

Tipepidine hydrochloride Chemical Structure

CAS No. : 1449686-84-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 72	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 72	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 285	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 430	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tipepidine hydrochloride:

Tipepidine Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Tipepidine hydrochloride reversibly inhibits dopamine (DA) D₂ receptor-mediated GIRK currents (I_DA(GIRK)) with an IC₅₀ of 7.0 μM. Tipepidine hydrochloride subsequently activates VTA dopamine neuron^[1]. Tipepidine hydrochloride, a non-narcotic antitussive, exerts an antidepressant-like effect^[2].

IC₅₀ & Target

IC50: 7.0 μM (dopamine D₂ receptor)^[1]

In Vivo

Tipepidine (i.p.; 10-40 mg/kg; 0.5-23 hours) significantly decreases the immobility time in the forced swimming test in ACTH-treated rats. Tipepidine (i.p.; 40 mg/kg) increases the extracellular dopamine level of the nucleus accumbens (NAc) in ACTH-treated rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats weighting 150-240 g (5-7 weeks old) ^[2]
Dosage:	10, 20 and 40 mg/kg
Administration:	I.p.; 0.5, 5, 23 hours
Result:	Decreased the immobility time in the forced swimming test in ACTH-treated rats.

Clinical Trial

Molecular Weight

311.89

Formula

C₁₅H₁₈ClNS₂

CAS No.

1449686-84-3

Appearance

Solid

Color

Light yellow to brown

SMILES

CN1C/C(CCC1)=C(C2=CC=CS2)\C3=CC=CS3.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 41.67 mg/mL (133.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 3.33 mg/mL (10.68 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2063 mL	16.0313 mL	32.0626 mL
5 mM	0.6413 mL	3.2063 mL	6.4125 mL
10 mM	0.3206 mL	1.6031 mL	3.2063 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 33.33 mg/mL (106.86 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (248 KB) LCMS (260 KB)

Handling Instructions (2659 KB)

References

[1]. Hamasaki R, et al. Tipepidine activates VTA dopamine neuron via inhibiting dopamine D₂ receptor-mediated inward rectifying K⁺ current. Neuroscience. 2013 Nov 12;252:24-34. [Content Brief]

[2]. Kawaura K, et al. Tipepidine, a non-narcotic antitussive, exerts an antidepressant-like effect in the forced swimming test in adrenocorticotropic hormone-treated rats. Behav Brain Res. 2016 Apr 1;302:269-78. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.2063 mL	16.0313 mL	32.0626 mL	80.1565 mL
	5 mM	0.6413 mL	3.2063 mL	6.4125 mL	16.0313 mL
	10 mM	0.3206 mL	1.6031 mL	3.2063 mL	8.0156 mL
DMSO	15 mM	0.2138 mL	1.0688 mL	2.1375 mL	5.3438 mL
	20 mM	0.1603 mL	0.8016 mL	1.6031 mL	4.0078 mL
	25 mM	0.1283 mL	0.6413 mL	1.2825 mL	3.2063 mL
	30 mM	0.1069 mL	0.5344 mL	1.0688 mL	2.6719 mL
	40 mM	0.0802 mL	0.4008 mL	0.8016 mL	2.0039 mL
	50 mM	0.0641 mL	0.3206 mL	0.6413 mL	1.6031 mL
	60 mM	0.0534 mL	0.2672 mL	0.5344 mL	1.3359 mL
	80 mM	0.0401 mL	0.2004 mL	0.4008 mL	1.0020 mL
	100 mM	0.0321 mL	0.1603 mL	0.3206 mL	0.8016 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.