1. Apoptosis
  2. Bcl-2 Family
  3. WEHI-539


Cat. No.: HY-15607
Handling Instructions

WEHI-539 is a selective inhibitor of Bcl-XL with an IC50 of 1.1 nM.

For research use only. We do not sell to patients.

WEHI-539 Chemical Structure

WEHI-539 Chemical Structure

CAS No. : 1431866-33-9

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Top Publications Citing Use of Products

    WEHI-539 purchased from MedChemExpress. Usage Cited in: Int J Cancer. 2018 Feb 1;142(3):584-596.  [Abstract]

    Evaluation of PARP protein cleavage in Mneo and MXL90 cells after treatment for 24h with 20 μM NSC 119875, in presence of increasing doses of the specific Bcl-xL inhibitor WEHI-539.

    WEHI-539 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2018 May 25;9(40):26046-26063.  [Abstract]

    Representative images of Hoechst/PI merged channels for WEHI-539 treatment in MCF7-pSFFV cell line.

    WEHI-539 purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2017 Jan;24(1):111-119.  [Abstract]

    BCL-XL-BIK complexes can be dissociated using the BCL-XL-inhibitor WEHI-539.

    WEHI-539 purchased from MedChemExpress. Usage Cited in: Blood. 2014 Dec 4;124(24):3587-96.  [Abstract]

    CD41+ or CD41 cells are treated with DMSO as a vehicle control or a BCL-xL inhibitor (WEHI-539; 0.2, 0.4, and 0.8 μM) on OP9 stromal cells in the presence of THPO for 7 days. The number of viable cells is counted.

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    WEHI-539 is a selective inhibitor of Bcl-XL with an IC50 of 1.1 nM.

    IC50 & Target[1]


    1.1 nM (IC50)

    In Vitro

    WEHI-539 is a selective inhibitor of Bcl-XL. WEHI-539 augments Carboplatin induced caspase 3/7 activity, PARP cleavage and annexin V labelling. WEHI-539 as a single agent causes noticeable PARP cleavage in Ovcar-4 (5 μM in Ovcar-4.) and Ovsaho (1 μM in Ovsaho) cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.



    Room temperature in continental US; may vary elsewhere.


    Please store the product under the recommended conditions in the Certificate of Analysis.

    Purity & Documentation
    Cell Assay

    Ovcar-8, Ovcar-3, Ovcar-4 and Ovsaho cells are grown in the RPMI, Igrov-1, Cov-362 and Cov-318 cells are grown in DMEM and Fuov-1 cells are grown in DMEM/F-12 nutrient mixture. ABT-737, ABT-199 and WEHI-539 (Medchem Express, NJ, USA), are prepared as a 20 mM solution in DMSO. For cell growth assays, cells are plated in 96 wells plate (5,000 cells/well for all cell lines except Ovcar-8 which is plated at a density of 2,500 cells/well). The next day, cells are treated with drugs. After 72 h the culture medium is removed and the cells are fixed with 100 μL of cold 10 % Trichloroacetic acid (TCA), incubated on ice for 30 min and stained with 0.4 % sulforhodamine B (SRB). The data are analysed by using Graphpad Prism 4 software. Non-linear regression is used to fit a four parameters Hill equation. For drug combinations studies the cells are exposed simultaneously to a range of concentrations of carboplatin combined with fixed concentration of BH3 mimetics that is expected from the single agent studies to cause 5 % growth inhibition: ABT-737, 1 μM in Ovcar-8, Ovcar-3 and Igrov-1, 2 μM in Ovcar-4 and Ovsaho and 6 μM in Cov-362; ABT-199, 1 μM in Ovcar-4, 2 μM in Ovcar-3, Igrov-1, Cov-362 and Ovsaho and 3 μM in Ovcar-8; WEHI-539, 0.2 μM in Igrov-1, 0.3 μM in Ovcar-8, 1 μM in Ovcar-3 and Ovsaho, 3.1 μM in Cov-362 and 5 μM in Ovcar-4. Surviving cell number is assessed by SRB staining. A combination index (CI) is calculated[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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