(24S,25)-Epoxycholesterol 

24S,25-Epoxycholesterol is an agonist for Liver X Receptor (LXR). 24S,25-Epoxycholesterol exhibits properties in regulating the cholesterol efflux^[1], inhibiting tumor growth against gastric cancer and glioblastoma^[2][3] and inducing apoptosis in BMMC cells^[5].

24S,25-Epoxycholesterol (1-10 μM) upregulates the LXR-related genes ABCA1, ABCG1 and APOE, promotes the cholesterol efflux, therefore prevents the foam cell formation and blocks proliferation of mouse and human glioma stem-like cells through depeting cellular cholesterol^[1][3].
24S,25-Epoxycholesterol inhibits proliferation and migration of HGC27, which is enhanced by knockout of LXRβ^[2].
24S,25-Epoxycholesterol (40 μM) inhibits HMG-CoA reductase and activates the LXR, which suppresses mevalonate-dependent isoprenoid production and enhancing the ATP-binding cassette G1 expression, induces apoptosis in bone marrow-derived murine mast cells (BMMCs)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

24S,25-Epoxycholesterol (5 mM, icv) enhances the mDA neurogenesis, limits the neurodegeneration in CYP46A1-overexpressing mice midbrain^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

400.64

C₂₇H₄₄O₂

77058-74-3

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CC[C@H]3C(C)(O3)C)([H])[C@@]4([H])[C@@](CC1)([H])[C@@]5(C(C[C@H](CC5)O)=CC4)C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Beyea MM, et al., Selective up-regulation of LXR-regulated genes ABCA1, ABCG1, and APOE in macrophages through increased endogenous synthesis of 24(S),25-epoxycholesterol. J Biol Chem. 2007 Feb 23;282(8):5207-16.  [Content Brief]

  [2]. Guo F, et al., Upregulation of 24(R/S),25-epoxycholesterol and 27-hydroxycholesterol suppresses the proliferation and migration of gastric cancer cells. Biochem Biophys Res Commun. 2018 Oct 12;504(4):892-898.  [Content Brief]

  [3]. Nguyen TP, et al., Selective and brain-penetrant lanosterol synthase inhibitors target glioma stem-like cells by inducing 24(S),25-epoxycholesterol production. Cell Chem Biol. 2023 Feb 16;30(2):214-229.e18.  [Content Brief]

  [4]. Theofilopoulos S, et al., 24(S),25-Epoxycholesterol and cholesterol 24S-hydroxylase (CYP46A1) overexpression promote midbrain dopaminergic neurogenesis in vivo. J Biol Chem. 2019 Mar 15;294(11):4169-4176.  [Content Brief]

  [5]. Fukunaga M, et al., Mast cell death induced by 24(S),25-epoxycholesterol. Exp Cell Res. 2010 Nov 15;316(19):3272-81.  [Content Brief]