2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. 2'-Deoxycytidine

2'-Deoxycytidine  (Synonyms: Deoxycytidine; Cytosine deoxyriboside; Deoxyribose cytidine)

Cat. No.: HY-D0184 Purity: 99.97%
COA Handling Instructions

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2'-Deoxycytidine, a deoxyribonucleoside, can inhibit biological effects of Bromodeoxyuridine (Brdu). 2'-Deoxycytidine is essential for the synthesis of nucleic acids, that can be used for the research of cancer.

For research use only. We do not sell to patients.

2'-Deoxycytidine Chemical Structure

2'-Deoxycytidine Chemical Structure

CAS No. : 951-77-9

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solid
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 2'-Deoxycytidine:

2'-Deoxycytidine Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

2'-Deoxycytidine, a deoxyribonucleoside, can inhibit biological effects of Bromodeoxyuridine (Brdu). 2'-Deoxycytidine is essential for the synthesis of nucleic acids, that can be used for the research of cancer[1][2].

IC50 & Target[1]

Brdu

 

Human Endogenous Metabolite

 

Microbial Metabolite

 

In Vivo

2'-Deoxycytidine (25 mg/kg, i.v., 4 times, from 8 days to 16 days) inhibits tumor growth in mice inoculated SP2/0 myeloma cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice inoculated SP2/0 myeloma cells[2]
Dosage: 25 mg/kg
Administration: i.v., 4 times, from 8 days to 16 days
Result: Decreased the tumor weight and volume.
Increased survival time.
Clinical Trial
Molecular Weight

227.22

Formula

C9H13N3O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N([C@H]2C[C@H](O)[C@@H](CO)O2)C=CC(N)=N1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (220.05 mM; Need ultrasonic)

DMSO : 50 mg/mL (220.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4010 mL 22.0051 mL 44.0102 mL
5 mM 0.8802 mL 4.4010 mL 8.8020 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 110 mg/mL (484.11 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.97%

COA (252 KB) HNMR (121 KB) RP-HPLC (189 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.4010 mL 22.0051 mL 44.0102 mL 110.0255 mL
5 mM 0.8802 mL 4.4010 mL 8.8020 mL 22.0051 mL
10 mM 0.4401 mL 2.2005 mL 4.4010 mL 11.0026 mL
15 mM 0.2934 mL 1.4670 mL 2.9340 mL 7.3350 mL
20 mM 0.2201 mL 1.1003 mL 2.2005 mL 5.5013 mL
25 mM 0.1760 mL 0.8802 mL 1.7604 mL 4.4010 mL
30 mM 0.1467 mL 0.7335 mL 1.4670 mL 3.6675 mL
40 mM 0.1100 mL 0.5501 mL 1.1003 mL 2.7506 mL
50 mM 0.0880 mL 0.4401 mL 0.8802 mL 2.2005 mL
60 mM 0.0734 mL 0.3668 mL 0.7335 mL 1.8338 mL
80 mM 0.0550 mL 0.2751 mL 0.5501 mL 1.3753 mL
100 mM 0.0440 mL 0.2201 mL 0.4401 mL 1.1003 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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2'-Deoxycytidine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2'-Deoxycytidine951-77-9Deoxycytidine Cytosine deoxyriboside Deoxyribose cytidineEndogenous Metabolitedeoxyribonucleosidecancernucleic acidsBrduInhibitorinhibitorinhibit

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