2. Protein Tyrosine Kinase/RTK
  3. FLT3
  4. 5'-Fluoroindirubinoxime

5'-Fluoroindirubinoxime  (Synonyms: 5'-FIO)

Cat. No.: HY-103464 Purity: ≥98.0%
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5'-Fluoroindirubinoxime (5’-FIO, compound 13), an Indirubin (HY-N0117) derivative, is a potent FLT3 inhibitor, with an IC50 of 15 nM.

For research use only. We do not sell to patients.

5'-Fluoroindirubinoxime Chemical Structure

5'-Fluoroindirubinoxime Chemical Structure

CAS No. : 861214-33-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 239 In-stock
Solution
10 mM * 1 mL in DMSO USD 239 In-stock
Solid
5 mg USD 218 In-stock
10 mg USD 350 In-stock
50 mg USD 550 In-stock
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Based on 1 publication(s) in Google Scholar

5'-Fluoroindirubinoxime Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

5'-Fluoroindirubinoxime (5’-FIO, compound 13), an Indirubin (HY-N0117) derivative, is a potent FLT3 inhibitor, with an IC50 of 15 nM[1].

IC50 & Target

15 nM (FLT3)[1].
In Vitro

5'-Fluoroindirubinoxime (5’-FIO, compound 13) exhibits IC50 values of 1.53 μM and 1.27 μM for VEGFR2 and Aurora A, respectively[1].
5'-Fluoroindirubinoxime (5’-FIO) exhibits IC50 values of 12.2 μM, 2.1 μM, 3.4 μM and 5.1 μM in A549, SNU-638, HT-1080 and RK3E-ras cancer cells, respectively. 5'-Fluoroindirubinoxime (5’-FIO) induces the apoptosis in RK3E-ras cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

5'-Fluoroindirubinoxime Related Antibodies
In Vivo

5'-Fluoroindirubinoxime (5’-FIO, 10 μmol/L/100 μL (~2.95 mg/mL) every other day beginning on day 6, S.C..) exhibits significant anti-tumor activity in rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rat tumor model based RK3E-ras cells[2].
Dosage: S.C..
Administration: 10 μmol/L/100 μL (~2.95 mg/mL) every other day beginning on day 6.
Result: Effectively inhibited tumor growth.
Molecular Weight

295.27

Formula

C16H10FN3O2
CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

O=C1NC2=C(C=C(F)C=C2)/C1=C3NC4=C(C=CC=C4)C\3=N/O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (282.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3867 mL 16.9337 mL 33.8673 mL
5 mM 0.6773 mL 3.3867 mL 6.7735 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.04 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3867 mL 16.9337 mL 33.8673 mL 84.6683 mL
5 mM 0.6773 mL 3.3867 mL 6.7735 mL 16.9337 mL
10 mM 0.3387 mL 1.6934 mL 3.3867 mL 8.4668 mL
15 mM 0.2258 mL 1.1289 mL 2.2578 mL 5.6446 mL
20 mM 0.1693 mL 0.8467 mL 1.6934 mL 4.2334 mL
25 mM 0.1355 mL 0.6773 mL 1.3547 mL 3.3867 mL
30 mM 0.1129 mL 0.5645 mL 1.1289 mL 2.8223 mL
40 mM 0.0847 mL 0.4233 mL 0.8467 mL 2.1167 mL
50 mM 0.0677 mL 0.3387 mL 0.6773 mL 1.6934 mL
60 mM 0.0564 mL 0.2822 mL 0.5645 mL 1.4111 mL
80 mM 0.0423 mL 0.2117 mL 0.4233 mL 1.0584 mL
100 mM 0.0339 mL 0.1693 mL 0.3387 mL 0.8467 mL
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5'-Fluoroindirubinoxime Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5'-Fluoroindirubinoxime861214-33-75'-FIOFLT3Cluster of differentiation antigen 135CD135Fms like tyrosine kinase 3A549SNU-638HT-1080RK3E-rasInhibitorinhibitorinhibit

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