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  5. Butylphthalide

Butylphthalide  (Synonyms: 3-n-Butylphthalide; 3-Butylphthalide)

Cat. No.: HY-B0647 Purity: 99.98%
COA Handling Instructions

Butylphthalide (3-n-Butylphthalide) is an active molecule against cerebral ischemia. It was originally isolated from celery species and has been shown to be effective in stroke animal models.

For research use only. We do not sell to patients.

Butylphthalide Chemical Structure

Butylphthalide Chemical Structure

CAS No. : 6066-49-5

Size Price Stock Quantity
Liquid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 106 In-stock
Solution
10 mM * 1 mL in DMSO USD 106 In-stock
Liquid
500 mg USD 60 In-stock
1 g USD 115 In-stock
5 g   Get quote  
10 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Butylphthalide:

Top Publications Citing Use of Products

    Butylphthalide purchased from MedChemExpress. Usage Cited in: Front Aging Neurosci. 2019 Feb 28;11:44.  [Abstract]

    The protein level of TNF-α, IL-1β, iNOS and COX2 is analyzed by Western Blot with or without the treatment of NBP and LPS.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Butylphthalide (3-n-Butylphthalide) is an active molecule against cerebral ischemia. It was originally isolated from celery species and has been shown to be effective in stroke animal models.

    In Vivo

    Butylphthalide (160mg/kg, i.g) improves learning and memory deficits in SAMP8 mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Old male SAMP8 mice and SAMR1 mice[3]
    Dosage: 40 mg/kg, 80 mg/kg, 160 mg/kg
    Administration: Orally daily, 60 days
    Result: Shorted escape latency time on day 7.
    Improved SAMP8 mice learning deficits.
    Clinical Trial
    Molecular Weight

    190.24

    Formula

    C12H14O2

    CAS No.
    Appearance

    Liquid

    Color

    Colorless to light yellow

    SMILES

    O=C1OC(CCCC)C2=C1C=CC=C2

    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Pure form -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (1314.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (525.65 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.2565 mL 26.2826 mL 52.5652 mL
    5 mM 1.0513 mL 5.2565 mL 10.5130 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (10.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 13.33 mg/mL (70.07 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.98%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.2565 mL 26.2826 mL 52.5652 mL 131.4129 mL
    5 mM 1.0513 mL 5.2565 mL 10.5130 mL 26.2826 mL
    10 mM 0.5257 mL 2.6283 mL 5.2565 mL 13.1413 mL
    15 mM 0.3504 mL 1.7522 mL 3.5043 mL 8.7609 mL
    20 mM 0.2628 mL 1.3141 mL 2.6283 mL 6.5706 mL
    25 mM 0.2103 mL 1.0513 mL 2.1026 mL 5.2565 mL
    30 mM 0.1752 mL 0.8761 mL 1.7522 mL 4.3804 mL
    40 mM 0.1314 mL 0.6571 mL 1.3141 mL 3.2853 mL
    50 mM 0.1051 mL 0.5257 mL 1.0513 mL 2.6283 mL
    60 mM 0.0876 mL 0.4380 mL 0.8761 mL 2.1902 mL
    80 mM 0.0657 mL 0.3285 mL 0.6571 mL 1.6427 mL
    100 mM 0.0526 mL 0.2628 mL 0.5257 mL 1.3141 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Butylphthalide
    Cat. No.:
    HY-B0647
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