C3a (70-77) (Synonyms: Complement 3a (70-77))

Cat. No.: HY-P1505: FAQs
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C3a (70-77) is an octapeptide corresponding to the COOH terminus of C3a, exhibits the specificity and 1 to 2% biologic activities of C3a.

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C3a (70-77) Chemical Structure

CAS No. : 63555-63-5

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Other In-stock Forms of C3a (70-77):

C3a (70-77) (TFA)

Other Forms of C3a (70-77):

C3a (70-77) (TFA) In-stock

1 Publications Citing Use of MCE C3a (70-77)

•Research Square Preprint. 2020 Dec.

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Description	C3a (70-77) is an octapeptide corresponding to the COOH terminus of C3a, exhibits the specificity and 1 to 2% biologic activities of C3a.
IC₅₀ & Target	Complement system^[1]
In Vitro	The direct interaction of C3a(70-77) with human mononuclear leukocytes in culture results in a concentration-dependent inhibition of the generation of LIF evoked by mitogens and by the antigen SK-SD. The extent of suppression of LIF generation by C3a(70-77) is significant at concentrations of 10^-7 M or higher and exceeds 75% at 10^-6 M, irrespective of the stimulus^[1]. C3a (70-77) induces histamine release and degranulation of rat mast cells, promotes contraction of guinea pig ileal tissue, and enhances vascular permeability in human skin. C3a (70-77) selectively desensitizes ileal smooth muscle to C3a but not to human C5a, a related anaphylatoxin. Conversely, C3a-(70-77) is unable to contract ileal smooth muscle pretreated with natural C3a. This cross-desensitization indicates a specific interaction of C3a (70-77) with cellular C3a binding sites^[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. C3a (70-77) Related Antibodies
Molecular Weight	823.94
Formula	C₃₅H₆₁N₁₃O₁₀
CAS No.	63555-63-5
Sequence	Ala-Ser-His-Leu-Gly-Leu-Ala-Arg
Sequence Shortening	ASHLGLAR
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (263 KB) Handling Instructions (2659 KB)
References	[1]. Payan DG, et al. Modulation of human lymphocyte function by C3a and C3a(70-77). J Exp Med. 1982 Sep 1;156(3):756-65. [Content Brief] [2]. Caporale LH, et al. The active site of C3a anaphylatoxin. J Biol Chem. 1980 Nov 25;255(22):10758-63. [Content Brief]

Kinase Assay
^[1]

2×10⁵ mononuelear leukocytes in 0.2 mL of M199-HPS containing 15% (vol/vol) AB-positive serum are added to each well of microtiter plates without or with 10 μg/mL of Con A, PHA, or SK-SD and incubated at 37°C in 5% CO₂:95% air. C3a or C3a(70-77) is introduced into some suspensions at the same time as the stimulus or buffer. After 3 d for the mitogen-stimulated cultures and 5 d for the SK-SD-stimulated cultures, 1 μCi of [³H]thymidine is added to each well. After an additional 16 h at 37°C, the uptake of [³H]thymidine is analyzed by aspirating the contents of each well with a Mash II harvester that collected and ished the leukocytes on glass fiber filters. The radioactivity in each filter is quantified, and the results for quadruplicate chambers are expressed as mean cpm+SEM^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Payan DG, et al. Modulation of human lymphocyte function by C3a and C3a(70-77). J Exp Med. 1982 Sep 1;156(3):756-65. [Content Brief]

[2]. Caporale LH, et al. The active site of C3a anaphylatoxin. J Biol Chem. 1980 Nov 25;255(22):10758-63. [Content Brief]

C3a (70-77) Related Classifications

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C3a (70-77)63555-63-5Complement 3a (70-77)Complement SystemInhibitorinhibitorinhibit

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