2. Metabolic Enzyme/Protease Apoptosis Autophagy
  3. Phosphatase Apoptosis Autophagy
  4. CCT007093

CCT007093 is an effective protein phosphatase 1D (PPM1D Wip1) inhibitor. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy.

For research use only. We do not sell to patients.

CCT007093 Chemical Structure

CCT007093 Chemical Structure

CAS No. : 176957-55-4

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10 mM * 1 mL in DMSO
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Based on 2 publication(s) in Google Scholar

CCT007093 Related Antibodies

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Description

CCT007093 is an effective protein phosphatase 1D (PPM1D Wip1) inhibitor. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy[1][2].

In Vitro

CCT007093 (25 or 50 µM, 8 h) significantly increases the phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159, and the phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) are also up-regulated in transfected HEK293T cell line[1].
CCT007093 shows specificity for MCF-7 cells, reducing viability by 40% after 2 days with no observable effect on the growth of HeLa cells[2].
CCT007093 induces P38 phosphorylation at 4 h post exposure in MCF-7 cells (sensitive to PPM1D inhibition), and does not induce P38 phosphorylation in HeLa cells (relatively resistant to PPM1D inhibition)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CCT007093 Related Antibodies

Western Blot Analysis[1]

Cell Line: HEK293T cell line.
Concentration: 25 or 50 µM.
Incubation Time: 8 h.
Result: The phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159 were all significantly increased[1].
The phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) were also up-regulated[1].
In Vivo

CCT007093 (6.4 mg/kg) enhances liver regeneration and increases the survival rate of mice following major hepatectomy[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type mice[1].
Dosage: 3.2 and 6.4 mg/kg.
Administration: Injected intraperitoneally 4 times (mice were sacrificed at 36 h post-PH).
Result: Significantly improved survival in mice following major hepatectomy (80% hepatectomy).
The level of PCNA was also significantly increased in the liver of CCT007093-treated mice at 36h, 48h and 72h post-PH.
Molecular Weight

272.39

Formula

C15H12OS2
CAS No.
Appearance

Solid

SMILES

O=C1/C(CC/C1=C\C2=CC=CS2)=C/C3=CC=CS3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMF : 3.33 mg/mL (12.23 mM; Need ultrasonic)

DMSO : ≥ 2.8 mg/mL (10.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6712 mL 18.3560 mL 36.7121 mL
5 mM 0.7342 mL 3.6712 mL 7.3424 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation

Purity: 98.01%

COA (249 KB) HNMR (245 KB) LCMS (258 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / DMF 1 mM 3.6712 mL 18.3560 mL 36.7121 mL 91.7802 mL
5 mM 0.7342 mL 3.6712 mL 7.3424 mL 18.3560 mL
10 mM 0.3671 mL 1.8356 mL 3.6712 mL 9.1780 mL
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CCT007093 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CCT007093176957-55-4CCT 007093CCT-007093PhosphataseApoptosisAutophagyInhibitorinhibitorinhibit

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