2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Campesterol

Campesterol  (Synonyms: (24R)-5-Ergosten-3β-ol)

Cat. No.: HY-N1459 Purity: 98.16%
COA Handling Instructions

Molarity Calculator

Campesterol is a plant sterol with cholesterol lowering and anticarcinogenic effects.

For research use only. We do not sell to patients.

Campesterol Chemical Structure

Campesterol Chemical Structure

CAS No. : 474-62-4

Size Price Stock Quantity
2 mg USD 79 In-stock
5 mg USD 158 In-stock
10 mg USD 238 In-stock
25 mg USD 502 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Campesterol:

Campesterol Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Campesterol

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Campesterol is a plant sterol with cholesterol lowering and anticarcinogenic effects.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Campesterol shows a weak cytotoxicity in non-proliferating human umbilical vein endothelial cells (HUVECs). Within the non-cytotoxic concentration range, campesterol significantly inhibits the bFGF-induced proliferation and tube formation of HUVECs in a concentration-dependent manner, while it does not affect the motility of HUVECs. 50 μg/mL of campesterol decreases the cell viability up to about 56% of control(IC50 of over 50 μg/mL)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Campesterol Related Antibodies
In Vivo

Campesterol effectively disrupts the bFGF-induced neovascularization in chick chorioallantoic membrane (CAM) in vivo[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

400.68

Formula

C28H48O
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@H](C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

Ethanol : 9.09 mg/mL (22.69 mM; Need ultrasonic)

H2O : 1 mg/mL (2.50 mM; ultrasonic and warming and heat to 80°C)

DMSO : 0.67 mg/mL (1.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*Campesterol is usually formulated as a suspension.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4958 mL 12.4788 mL 24.9576 mL
5 mM 0.4992 mL 2.4958 mL 4.9915 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: 0.91 mg/mL (2.27 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 0.91 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (9.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 0.91 mg/mL (2.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 0.91 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (9.1 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.16%

COA (249 KB) RP-HPLC (245 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

The effect of campesterol on the viable cell number was assessed by XTT assay. HUVECs were treated with various concentrations (10, 20, 30, 40 and 50 μg/mL) of campesterol and incubated at 37 °C in a humidified incubator for 24 h, a XTT working solution was added to each well. The cells were incubated at 37 °C for 2 h and the optical density was measured using a microplate reader at 450 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O / Ethanol 1 mM 2.4958 mL 12.4788 mL 24.9576 mL 62.3939 mL
Ethanol 5 mM 0.4992 mL 2.4958 mL 4.9915 mL 12.4788 mL
10 mM 0.2496 mL 1.2479 mL 2.4958 mL 6.2394 mL
15 mM 0.1664 mL 0.8319 mL 1.6638 mL 4.1596 mL
20 mM 0.1248 mL 0.6239 mL 1.2479 mL 3.1197 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Campesterol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Campesterol474-62-4(24R)-5-Ergosten-3β-olEndogenous MetaboliteInhibitorinhibitorinhibit

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