Castanospermine

Name: Castanospermine
Brand: MedChemExpress
SKU: HY-N2022
Rating: 4.5 (2 reviews)

Cat. No.: HY-N2022 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Castanospermine is a natural alkaloid that can be extracted from black beans or the Moreton Bay chestnut tree (Castanospermum australae). Castanospermine is an α/ β-glucosidase inhibitor. Castanospermine has anti-inflammatory, antiviral replication and anti-metastatic effects on prostate cancer. Castanospermine can be used as an immunosuppressant to prevent transplant rejection.

For research use only. We do not sell to patients.

Castanospermine Chemical Structure

CAS No. : 79831-76-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 42	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 106	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Castanospermine

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Castanospermine (0.01-1000 μM, 48 h) inhibits the production of DEN infectious virus in a dose-dependent manner and slows the electrophoretic mobility of DEN prM (a glycosylated structural protein) in BGK-21 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	BHK-21
Concentration:	100, 500 μM
Incubation Time:	48 h
Result:	Reduced marker gene expression or propagation of WNV or DEN replicons modestly by up to 20 to 40%, respectively. Had small effects on the secretion of infectious WNV or viral particles containing WNV RNA but decreased the amount of DEN viral RNA and infectious virus.

In Vivo

Castanospermine (10, 50, 250 mg/kg, i.p., a single dose for 10 consecutive days) can improve the survival rate of mice infected with DEN-2 virus^[1]. Castanospermine (10-500 mg/kg, intraperitoneal injection) can improve the symptoms of acute pancreatic injury in rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	A/J mice model^[1]
Dosage:	10, 50, 250 mg/kg
Administration:	i.p.
Result:	Decreased secretion and virus infectivity, and increased survival rate of mice infected with DEN virus.

Animal Model:	Acute pancreatitis (AP) rat model^[2]
Dosage:	10, 50, 100, 200, 500 mg/kg
Administration:	i.p.
Result:	The extent and severity of the pancreatic injury were significantly decreased. Decreased the interleukin production in serum and NF-kB activation. Increased the level of TNF-a, ICAM-1 and VCAM-1.

Molecular Weight

189.21

Formula

C₈H₁₅NO₄

CAS No.

79831-76-8

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](O)[C@H](O)[C@]([C@@H](O)CC2)([H])N2C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (528.51 mM; Need ultrasonic)

DMSO : 100 mg/mL (528.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2851 mL	26.4257 mL	52.8513 mL
5 mM	1.0570 mL	5.2851 mL	10.5703 mL
10 mM	0.5285 mL	2.6426 mL	5.2851 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 120 mg/mL (634.22 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (282 KB) SDS (0 KB)

COA (254 KB) HNMR (237 KB)

Handling Instructions (2659 KB)

References

[1]. Whitby K, et al. Castanospermine, a potent inhibitor of dengue virus infection in vitro and in vivo. J Virol. 2005 Jul;79(14):8698-706. [Content Brief]

[2]. Hong YP, et al. Effects of Castanospermine on Inflammatory Response in a Rat Model of Experimental Severe Acute Pancreatitis. Arch Med Res. 2016 Aug;47(6):436-445. [Content Brief]

[3]. Elbein AD. Glycosidase inhibitors: inhibitors of N-linked oligosaccharide processing. FASEB J. 1991 Dec;5(15):3055-63 [Content Brief]

[4]. den Dulk M, et al. Combined donor leucocyte administration and immunosuppressive drug treatment for survival of rat heart allografts. Transpl Immunol. 2004 Nov;13(3):177-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.2851 mL	26.4257 mL	52.8513 mL	132.1283 mL
	5 mM	1.0570 mL	5.2851 mL	10.5703 mL	26.4257 mL
	10 mM	0.5285 mL	2.6426 mL	5.2851 mL	13.2128 mL
	15 mM	0.3523 mL	1.7617 mL	3.5234 mL	8.8086 mL
	20 mM	0.2643 mL	1.3213 mL	2.6426 mL	6.6064 mL
	25 mM	0.2114 mL	1.0570 mL	2.1141 mL	5.2851 mL
	30 mM	0.1762 mL	0.8809 mL	1.7617 mL	4.4043 mL
	40 mM	0.1321 mL	0.6606 mL	1.3213 mL	3.3032 mL
	50 mM	0.1057 mL	0.5285 mL	1.0570 mL	2.6426 mL
	60 mM	0.0881 mL	0.4404 mL	0.8809 mL	2.2021 mL
	80 mM	0.0661 mL	0.3303 mL	0.6606 mL	1.6516 mL
	100 mM	0.0529 mL	0.2643 mL	0.5285 mL	1.3213 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.