2. MAPK/ERK Pathway
  3. JNK
  4. DB07268

DB07268 is a potent and selective JNK1 inhibitor with an IC50 value of 9 nM.

For research use only. We do not sell to patients.

DB07268 Chemical Structure

DB07268 Chemical Structure

CAS No. : 929007-72-7

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 74 In-stock
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10 mM * 1 mL in DMSO USD 74 In-stock
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5 mg USD 105 In-stock
10 mg USD 165 In-stock
50 mg USD 605 In-stock
100 mg USD 957 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

DB07268 Related Antibodies

Top Publications Citing Use of Products

    DB07268 purchased from MedChemExpress. Usage Cited in: Toxins. 2021 Mar 18;13(3):221.  [Abstract]

    The JNK1 inhibitor DB07268 reduces the combination-induced cell death significantly. Consistent with the reduction in apoptosis, a significantly decreased ROS level is detected by pretreatment with DB07268. Meanwhile, the activation of p53 and Bim induced by the combination is nearly reduced to control level.

    View All JNK Isoform Specific Products:

    View All Isoforms
    JNK1 JNK2 JNK3 JNK

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    DB07268 is a potent and selective JNK1 inhibitor with an IC50 value of 9 nM.

    IC50 & Target[1]

    JNK1

    9 nM (IC50)

    CHK1

    0.82 μM (IC50)

    PAK4

    5.5 μM (IC50)

    AKT1

    15 μM (IC50)

    ERK2

    25 μM (IC50)

    In Vitro

    DB07268 (Compound 2b) also inhibits CHK1, PAK4, AKT1, and ERK2 with IC50s of 0.82 μM, 5.5 μM, 15 μM, and 25 μM, respectively[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DB07268 Related Antibodies
    Molecular Weight

    321.33

    Formula

    C17H15N5O2
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(N)C1=CC=CC=C1NC2=NC(NC3=CC=CC(O)=C3)=NC=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 32 mg/mL (99.59 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1121 mL 15.5603 mL 31.1207 mL
    5 mM 0.6224 mL 3.1121 mL 6.2241 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.47 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.47 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.92%

    COA (250 KB) LCMS (124 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1121 mL 15.5603 mL 31.1207 mL 77.8016 mL
    5 mM 0.6224 mL 3.1121 mL 6.2241 mL 15.5603 mL
    10 mM 0.3112 mL 1.5560 mL 3.1121 mL 7.7802 mL
    15 mM 0.2075 mL 1.0374 mL 2.0747 mL 5.1868 mL
    20 mM 0.1556 mL 0.7780 mL 1.5560 mL 3.8901 mL
    25 mM 0.1245 mL 0.6224 mL 1.2448 mL 3.1121 mL
    30 mM 0.1037 mL 0.5187 mL 1.0374 mL 2.5934 mL
    40 mM 0.0778 mL 0.3890 mL 0.7780 mL 1.9450 mL
    50 mM 0.0622 mL 0.3112 mL 0.6224 mL 1.5560 mL
    60 mM 0.0519 mL 0.2593 mL 0.5187 mL 1.2967 mL
    80 mM 0.0389 mL 0.1945 mL 0.3890 mL 0.9725 mL
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    DB07268 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DB07268929007-72-7DB 07268DB-07268JNKc-Jun N-terminal kinaseInhibitorinhibitorinhibit

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