2. Metabolic Enzyme/Protease Apoptosis
  3. MMP Apoptosis
  4. Edaravone

Edaravone is a strong novel free radical scavenger, and inhibits MMP-9-related brain hemorrhage in rats treated with tissue plasminogen activator.

For research use only. We do not sell to patients.

Edaravone Chemical Structure

Edaravone Chemical Structure

CAS No. : 89-25-8

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 61 In-stock
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10 mM * 1 mL in DMSO USD 61 In-stock
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10 g USD 38 In-stock
25 g USD 58 In-stock
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Customer Review

Based on 22 publication(s) in Google Scholar

Other Forms of Edaravone:

Edaravone Related Antibodies

Top Publications Citing Use of Products

    Edaravone purchased from MedChemExpress. Usage Cited in: Int Wound J. 2023 Apr 11.  [Abstract]

    Edaravone (EDA; 20.0 mg/kg/day; i.p.; 7 days) significantly promotes VEGF and MMP9 expression in mice.

    Edaravone purchased from MedChemExpress. Usage Cited in: Int Wound J. 2023 Apr 11.  [Abstract]

    Edaravone (EDA; 20.0 mg/kg/day; i.p.; 7 days) significantly reduces the number of apoptotic cells in mice.

    Edaravone purchased from MedChemExpress. Usage Cited in: Int Wound J. 2023 Apr 11.  [Abstract]

    Edaravone (EDA; 20.0 mg/kg/day; i.p.; 7 days) significantly increases the protein expression of TGF-β1, VEGF and MMP9 in mice.

    Edaravone purchased from MedChemExpress. Usage Cited in: Front Physiol. 2020 Jan 15;10:1596.

    A) HE staining of muscle tissue after artery ligation shows that the cells are round, and reveals infiltration of inflammatory cells in both control (Con) and ob/ob (Ob) mice. (B) MyoD immunostaining is lower in Ob than Con at 7 days after ligation of the femoral artery. However, pretreatment with Edaravone increases the expression of MyoD in obese mice.

    Edaravone purchased from MedChemExpress. Usage Cited in: Front Physiol. 2020 Jan 15;10:1596.

    TNF-α expression is significantly increased by treating C2C12 cells with 250 µM H2O2. In addition, pretreatment with edaravone at 100 µM, TNF-α expression is significantly suppressed (∗ compared with 0 µM H2O2, p < 0.01; # compared with 250 µM H2O2, p < 0.01).

    Edaravone purchased from MedChemExpress. Usage Cited in: Front Physiol. 2020 Jan 15;10:1596.

    After treating C2C12 cells with 250 µM H2O2, viability is significantly decreased, and improvement in survival rate was observed in the group treated with Edaravone at 100 µM (∗compared with 0 µM H2O2, p < 0.01; # compared with 250 µM H2O2, p < 0.01).

    View All MMP Isoform Specific Products:

    View All Isoforms
    MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Edaravone is a strong novel free radical scavenger, and inhibits MMP-9-related brain hemorrhage in rats treated with tissue plasminogen activator.

    In Vitro

    Edaravone performs both preventative and therapeutic effects against toxicity of glutamate. Pretreatment of edaravone reduces the toxicity of glutamate towards SGNs. Edaravone reduces apoptosis and necrosis caused by glutamate. Pretreatment of edaravone (500 μM) reverses these changes to approximately normal levels. The protective effect of edaravone on SGNs against glutamate-induced apoptosis is associated with PI3K/Akt pathway and Bcl-2 protein family[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Edaravone Related Antibodies
    In Vivo

    Edaravone exerts neuroprotective effects by inhibiting endothelial injury and by ameliorating neuronal damage in brain ischemia. Edaravone provides the desirable features of NOS: it increases eNOS (beneficial NOS for rescuing ischemic stroke) and decreases nNOS and iNOS (detrimental NOS). Post-reperfusion brain edema and hemorrhagic events induced by thrombolytic therapy may be reduced by edaravone pretreatment[1]. Edaravone significantly decreases infarct volume, with the average infarct volume in the edaravone-treated rats (227.6 mm3) being significantly lower than that in the control rats (264.0 mm3). Edaravone treatment also decreases the postischemic hemorrhage volumes (53.4 mm3 in edaravone-treated rats vs 176.4 mm3 in controls). In addition, the ratio of hemorrhage volume to infarct volume is lower in the edaravone-treated rats (23.5%) than in the untreated rats (63.2%)[2]. In edaravone (20 mg/kg)-treated rats, astrocyte activity (glial fibrillary acidic protein) and apoptotic cells (caspase-3) are decreased on the corpus callosum, germinal matrix, and cerebral cortex[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    174.20

    Formula

    C10H10N2O
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1N(C2=CC=CC=C2)N=C(C)C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (574.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7405 mL 28.7026 mL 57.4053 mL
    5 mM 1.1481 mL 5.7405 mL 11.4811 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (14.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (14.35 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.83%

    COA (253 KB) HNMR (245 KB) RP-HPLC (206 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [4]

    Cell viability is quantified by MTT assay and trypan blue staining. MTT (5 mg/mL, 20 μL) is added to each well and incubated for 4 h at 37°C after the drug treatments. The medium is removed and the cell pellet is dissolved in DMSO. Then, the optical density (OD) values are measured at 570 nm using an ELISA reader.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 5.7405 mL 28.7026 mL 57.4053 mL 143.5132 mL
    5 mM 1.1481 mL 5.7405 mL 11.4811 mL 28.7026 mL
    10 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
    15 mM 0.3827 mL 1.9135 mL 3.8270 mL 9.5675 mL
    20 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
    25 mM 0.2296 mL 1.1481 mL 2.2962 mL 5.7405 mL
    30 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
    40 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
    50 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
    60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
    80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
    100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL
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    Edaravone Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Edaravone89-25-8MCI-186MCI186MCI 186MMPApoptosisMatrix metalloproteinasesInhibitorinhibitorinhibit

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