Engeletin

Name: Engeletin
Brand: MedChemExpress
SKU: HY-N0436
Rating: 4.5 (4 reviews)

Cat. No.: HY-N0436 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Engeletin is a flavanonol glycoside isolated from Smilax glabra Roxb. , inhibits NF-κB signaling-pathway activation, and possesses anti-inflammatory, analgesic, diuresis, detumescence, and antibiosis effects.

For research use only. We do not sell to patients.

Engeletin Chemical Structure

CAS No. : 572-31-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 23	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

3 Publications Citing Use of MCE Engeletin

: Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9;14:745-760. [Abstract]; Chondrocytes are pretreated with Engeletin (10 μM and 20 μM) for two hours, and then TNF-α (20 ng/mL) is added to the culture medium and incubated for another 24 hours. The protein expression levels of collagen II, aggrecan, MMP9 and MMP3 are determined by Western blotting.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

NF-κB

In Vitro

Engeletin is a flavanonol glycoside isolated from hymenaea martiana, inhibits NF-κB signaling-pathway activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Engeletin (25, 50, 100 mg/kg, i.p.) markedly reduces LPS-increased myeloperoxidase activity in mice, activates NF-κB-pathway activation, decreases the production of inflammatory mediators (iNOS and COX-2), and suppresses the expression of TLR4-signaling downstream molecules such as MyD88, IRAK1, TRAF6, and TAK1 proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

434.39

Formula

C₂₁H₂₂O₁₀

CAS No.

572-31-6

Appearance

Solid

Color

White to yellow

SMILES

OC1=CC(O)=C(C([C@H](O[C@@]2([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O2)[C@@H](C3=CC=C(O)C=C3)O4)=O)C4=C1

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (230.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3021 mL	11.5104 mL	23.0208 mL
5 mM	0.4604 mL	2.3021 mL	4.6042 mL
10 mM	0.2302 mL	1.1510 mL	2.3021 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (279 KB) SDS (392 KB)

COA (0 KB) HNMR (295 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Wu H, et al. Engeletin Alleviates Lipopolysaccharide-Induced Endometritis in Mice by Inhibiting TLR4-mediated NF-κB Activation. J Agric Food Chem. 2016 Aug 10;64(31):6171-8. [Content Brief]

Animal Administration
^[1]

Mice^[1]
The mice are classified at random into the following six groups of 10 mice each to cause the endometritis model: blank group, LPS group, Engeletin (25, 50, and 100 mg/kg) + LPS groups, and Engeletin (100 mg/kg) group. Engeletin is solubilized by heated normal saline to give the final concentrations of 25, 50, and 100 mg/kg. Briefly, each uterus is infused with 50 μL of LPS (1 mg/mL) to induce endometritis. At 24 h after the instillation, Engeletin groups receive an intraperitoneal injection of diverse Engeletin concentrations (25, 50, and 100 mg/kg) three times (once every 6 h). The Engeletin group is given an intraperitoneal injection of Engeletin (100 mg/kg). The blank group receive the normal saline. Afterward, the mice are killed by CO₂ inhalation. Uterus tissues are harvested and kept in −80°C^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Wu H, et al. Engeletin Alleviates Lipopolysaccharide-Induced Endometritis in Mice by Inhibiting TLR4-mediated NF-κB Activation. J Agric Food Chem. 2016 Aug 10;64(31):6171-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3021 mL	11.5104 mL	23.0208 mL	57.5520 mL
	5 mM	0.4604 mL	2.3021 mL	4.6042 mL	11.5104 mL
	10 mM	0.2302 mL	1.1510 mL	2.3021 mL	5.7552 mL
	15 mM	0.1535 mL	0.7674 mL	1.5347 mL	3.8368 mL
	20 mM	0.1151 mL	0.5755 mL	1.1510 mL	2.8776 mL
	25 mM	0.0921 mL	0.4604 mL	0.9208 mL	2.3021 mL
	30 mM	0.0767 mL	0.3837 mL	0.7674 mL	1.9184 mL
	40 mM	0.0576 mL	0.2878 mL	0.5755 mL	1.4388 mL
	50 mM	0.0460 mL	0.2302 mL	0.4604 mL	1.1510 mL
	60 mM	0.0384 mL	0.1918 mL	0.3837 mL	0.9592 mL
	80 mM	0.0288 mL	0.1439 mL	0.2878 mL	0.7194 mL
	100 mM	0.0230 mL	0.1151 mL	0.2302 mL	0.5755 mL

Engeletin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Engeletin572-31-6NF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Engeletin

Customer Review

Engeletin Related Antibodies

3 Publications Citing Use of MCE Engeletin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All NF-κB Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Engeletin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Engeletin Related Classifications

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