2. Anti-infection Cell Cycle/DNA Damage Epigenetics
  3. Bacterial DNA/RNA Synthesis MicroRNA Antibiotic
  4. Enoxacin hydrate

Enoxacin hydrate  (Synonyms: Enoxacin sesquihydrate; AT-2266 hydrate; CI-919 hydrate)

Cat. No.: HY-B0268A Purity: 99.85%
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Enoxacin hydrate (Enoxacin sesquihydrate), a fluoroquinolone, interferes with DNA replication and inhibits bacterial DNA gyrase (IC50=126 µg/ml) and topoisomerase IV (IC50=26.5 µg/ml). Enoxacin hydrate is a miRNA processing activator and enhances siRNA-mediated mRNA degradation and promotes the biogenesis of endogenous miRNAs. Enoxacin hydrate has potent activities against gram-positive and -negative bacteria. Enoxacin hydrate is a cancer-specific growth inhibitor that acts by enhancing TAR RNA-binding protein 2 (TRBP)-mediated microRNA processing.

For research use only. We do not sell to patients.

Enoxacin hydrate Chemical Structure

Enoxacin hydrate Chemical Structure

CAS No. : 84294-96-2

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Based on 1 publication(s) in Google Scholar

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Enoxacin hydrate Related Antibodies

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Description

Enoxacin hydrate (Enoxacin sesquihydrate), a fluoroquinolone, interferes with DNA replication and inhibits bacterial DNA gyrase (IC50=126 µg/ml) and topoisomerase IV (IC50=26.5 µg/ml). Enoxacin hydrate is a miRNA processing activator and enhances siRNA-mediated mRNA degradation and promotes the biogenesis of endogenous miRNAs. Enoxacin hydrate has potent activities against gram-positive and -negative bacteria. Enoxacin hydrate is a cancer-specific growth inhibitor that acts by enhancing TAR RNA-binding protein 2 (TRBP)-mediated microRNA processing[1][2][3][4].

IC50 & Target

Quinolone

 

In Vitro

Enoxacin hydrate (Enoxacin sesquihydrate) increases siGFP-mediated gene knockdown mediated by siRNA against EGFP in HEK293 cells-based reporter system in a dose-dependent manner, with a median effective concentration (EC50) of ~30 µM, whereas it has no effect on the cells expressing GFP only. Enoxacin (50 µM) promotes the processing of miRNAs and the loading of siRNA duplexes onto RISCs in HEK293 cells[3].
Enoxacin has no effect on the processing of pre-let-7 or pre-miR-30a by Dicer alone. However, the addition of Enoxacin can enhance the processing of let-7 or pre-miR-30a by Dicer and TRBP together[3].
Enoxacin inhibits 90% Escherichia coli, Klebsiella sp., Aeromonas sp., Enterobacter spp., Serratia spp., Proteus mirabilis, and Morganella morganii at less than or equal to 0.8 micrograms/ml[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Enoxacin hydrate Related Antibodies
In Vivo

Enoxacin hydrate (Enoxacin sesquihydrate; 100 µM; 2 µl; injected into ear once a day for 3 consecutive days (days 12, 13 and 14)) enhances the the GFP mRNA knockdown efficiency by Lv-siGFP (from 80% to 60%; 40% GFP mRNA level remained), whereas alone has no effect on GFP expression in GFP transgenic line C57BL/6-Tg(ACTB-EGFP)1Osb/J (10 d old) with lentivirus expressing shGFP (Lv-siGFP; injected into ear for 10 days)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

347.34

Formula

C15H17FN4O3.3/2H2O
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=CN(CC)C2=C(C=C(F)C(N3CCNCC3)=N2)C1=O)O.[1.5H2O]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

1M NaOH : 100 mg/mL (287.90 mM; ultrasonic and adjust pH to 11 with NaOH)

DMSO : 2.78 mg/mL (8.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8790 mL 14.3951 mL 28.7902 mL
5 mM 0.5758 mL 2.8790 mL 5.7580 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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V1

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.85%

COA (250 KB) HNMR (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / 1M NaOH 1 mM 2.8790 mL 14.3951 mL 28.7902 mL 71.9756 mL
5 mM 0.5758 mL 2.8790 mL 5.7580 mL 14.3951 mL
1M NaOH 10 mM 0.2879 mL 1.4395 mL 2.8790 mL 7.1976 mL
15 mM 0.1919 mL 0.9597 mL 1.9193 mL 4.7984 mL
20 mM 0.1440 mL 0.7198 mL 1.4395 mL 3.5988 mL
25 mM 0.1152 mL 0.5758 mL 1.1516 mL 2.8790 mL
30 mM 0.0960 mL 0.4798 mL 0.9597 mL 2.3992 mL
40 mM 0.0720 mL 0.3599 mL 0.7198 mL 1.7994 mL
50 mM 0.0576 mL 0.2879 mL 0.5758 mL 1.4395 mL
60 mM 0.0480 mL 0.2399 mL 0.4798 mL 1.1996 mL
80 mM 0.0360 mL 0.1799 mL 0.3599 mL 0.8997 mL
100 mM 0.0288 mL 0.1440 mL 0.2879 mL 0.7198 mL
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Enoxacin hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Enoxacin84294-96-2Enoxacin sesquihydrateAT-2266CI-919AT2266AT 2266CI919CI 919BacterialDNA/RNA SynthesisMicroRNAAntibioticmiRNADNAgyrasetopoisomerasesiRNAmRNAgram-positivegram-negativeTAR-RNATRBPInhibitorinhibitorinhibit

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