Ginsenoside Rh1 (Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)

Name: Ginsenoside Rh1
Brand: MedChemExpress
SKU: HY-N0604
Rating: 4.5 (2 reviews)

Cat. No.: HY-N0604 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ginsenoside Rh1 (Prosapogenin A2) inhibits the expression of PPAR-γ, TNF-α, IL-6, and IL-1β.

For research use only. We do not sell to patients.

Ginsenoside Rh1 Chemical Structure

CAS No. : 63223-86-9

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 56	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 56	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Ginsenoside Rh1:

(20R)-Ginsenoside Rh1 In-stock
Ginsenoside Rh1 (Standard) Get quote

2 Publications Citing Use of MCE Ginsenoside Rh1

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PPARα PPARβ/δ PPARγ PPAR PPARδ

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Ginsenoside Rh1 (Prosapogenin A2) inhibits the expression of PPAR-γ, TNF-α, IL-6, and IL-1β.

IC₅₀ & Target^[1]

PPAR-γ

IL-6

IL-1β

In Vitro

The effect of Ginsenoside Rh1 is examined on adipogenesis in 3T3-L1 cells. Ginsenoside Rh1 potently inhibits the adipogenesis, as assessed by Oil-red O staining and lipid contents in 3T3-L1 adipocytes. Ginsenoside Rh1, at concentrations of 50 μM and 100 μM, inhibit the adipogenesis by 50% and 63%, respectively.The expression levels of adipocytespecific genes such as PPAR-γ, C/EBP-α, FAS, aFABP and some genes are examined during early phase of differentiation such as Pref-1, C/EBP-δ and Glucocorticoid receptor (GR). After the treatment with Ginsenoside Rh1 in 3T3-L1 cells, mRNA is extracted on 18 h and 24 h for Pref-1, C/EBP-δ and GR and day 8 for PPAR-γ, C/EBP-α, FAS, aFABP. Then, the expression profiles of adipocyte-specific genes are investigated by RT-PCR. PPAR-γ, C/EBP-α, FAS, and aFABP expressions are significantly increased in DMI-stimulated differentiated adipocyte compared to those of non-stimulated adipocyte cells. However, treatment with DMI in the presence of Ginsenoside Rh1 significantly suppresses the expression levels of PPAR-γ, C/EBP-α, FAS, and aFABP in a dose- dependent manner, whereas the expression levels of Pref-1, C/EBP-δ and GR are not affected^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

When high-fat diet (HFD) fed mice for 8 weeks, body and epididymal fat weight gains are significantly increased compared to those of low-fat diet (LFD)-fed mice. However, when Ginsenoside Rh1 is treated in HFD-fed mice, body and epididymal fat weight gains are significantly decrease compared with those of the HFD-fed mice. TG, glucose, insulin, total cholesterol, and HDL levels in the blood are significantly increased in HFD-fed mice group compared to LFD-fed mice group. Treatment with Ginsenoside Rh1 in HFD-fed mice significantly lowers TG level alone^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

638.87

Formula

C₃₆H₆₂O₉

CAS No.

63223-86-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]([C@@]12C)(C[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)[C@@]4([H])C5(C)C)[C@@](C[C@@H](O)[C@]1([H])[C@]([C@@](C)(O)CC/C=C(C)/C)([H])CC2)([H])[C@]4(CC[C@@H]5O)C

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (156.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5653 mL	7.8263 mL	15.6526 mL
5 mM	0.3131 mL	1.5653 mL	3.1305 mL
10 mM	0.1565 mL	0.7826 mL	1.5653 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (301 KB) SDS (0 KB)

COA (258 KB) HNMR (326 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Gu W, et al. Ginsenoside Rh1 ameliorates high fat diet-induced obesity in mice by inhibiting adipocyte differentiation. Biol Pharm Bull. 2013;36(1):102-7. [Content Brief]

Cell Assay ^[1]	Mouse embryo fibroblasts 3T3-L1 cells are incubated in DMEM, containing 10% FBS and 1% AB, at 37°C and 5.6% CO2 atmosphere. To induce differentiation, two days after confluence, preadipocytes (designated day 0) are cultured in the differentiation medium (DM), which is consisted of DMEM, 10% FBS, 1% AB, and DMI (0.28 unit/mL insulin, 0.5 mM Isobutylmethylxanthine and 1 μM Dexamethasone) for 2 d in the presence or absence of 50 μM and 100 μM of Ginsenoside Rh1, and switched to DM containing 10% FBS and 10 μg/mL insulin and then changed to DMEM medium with 10% FBS for every 2 d^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] Male C57BL/6J mice are separated into 3 groups, LFD, HFD and HFDRh1. Each group is consisted of ten mice. LFD group fed LFD for 8 weeks. HFD group fed HFD for 8 weeks. HFDRh1 group fed HFD diet for 4 weeks and then simultaneously treated with HFD and 20 mg/kg/d Ginsenoside Rh1, which is orally administrated. Weight and food intake of mice are measured daily. After finishing treatment for 4 weeks, blood and epididymal fats are collected for further analysis. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Gu W, et al. Ginsenoside Rh1 ameliorates high fat diet-induced obesity in mice by inhibiting adipocyte differentiation. Biol Pharm Bull. 2013;36(1):102-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5653 mL	7.8263 mL	15.6526 mL	39.1316 mL
	5 mM	0.3131 mL	1.5653 mL	3.1305 mL	7.8263 mL
	10 mM	0.1565 mL	0.7826 mL	1.5653 mL	3.9132 mL
	15 mM	0.1044 mL	0.5218 mL	1.0435 mL	2.6088 mL
	20 mM	0.0783 mL	0.3913 mL	0.7826 mL	1.9566 mL
	25 mM	0.0626 mL	0.3131 mL	0.6261 mL	1.5653 mL
	30 mM	0.0522 mL	0.2609 mL	0.5218 mL	1.3044 mL
	40 mM	0.0391 mL	0.1957 mL	0.3913 mL	0.9783 mL
	50 mM	0.0313 mL	0.1565 mL	0.3131 mL	0.7826 mL
	60 mM	0.0261 mL	0.1304 mL	0.2609 mL	0.6522 mL
	80 mM	0.0196 mL	0.0978 mL	0.1957 mL	0.4891 mL
	100 mM	0.0157 mL	0.0783 mL	0.1565 mL	0.3913 mL

Ginsenoside Rh1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ginsenoside Rh1 (Synonyms: Prosapogenin A2; Sanchinoside B2; Sanchinoside Rh1)

Customer Review

Other Forms of Ginsenoside Rh1:

Ginsenoside Rh1 Related Antibodies

2 Publications Citing Use of MCE Ginsenoside Rh1

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•Related Screening Libraries:

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•Related Proteins:

View All PPAR Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Ginsenoside Rh1 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ginsenoside Rh1 Related Classifications

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