1. Anti-infection MAPK/ERK Pathway NF-κB Apoptosis
  2. Bacterial p38 MAPK NF-κB Apoptosis
  3. Hederacoside C

Hederacoside C  (Synonyms: Kalopanaxsaponin B)

Cat. No.: HY-N0253 Purity: 99.35%
COA Handling Instructions

Hederacoside C (Kalopanaxsaponin B) is an ingredient that can be obtained mainly from ivy leaves. Hederacoside C mediates inflammation by inhibiting activation of MAPK/NF-κB and its downstream signaling pathway. Hederacoside C has anti-inflammatory and antibacterial activity.

For research use only. We do not sell to patients.

Hederacoside C Chemical Structure

Hederacoside C Chemical Structure

CAS No. : 14216-03-6

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Solid + Solvent
10 mM * 1 mL in DMSO
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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
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25 mg USD 60 In-stock
50 mg USD 110 In-stock
100 mg USD 200 In-stock
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Description

Hederacoside C (Kalopanaxsaponin B) is an ingredient that can be obtained mainly from ivy leaves. Hederacoside C mediates inflammation by inhibiting activation of MAPK/NF-κB and its downstream signaling pathway. Hederacoside C has anti-inflammatory and antibacterial activity[1][2][3].

In Vitro

Hederacoside C (0.1, 1, 10 μM, 1 h) inhibits MAPK/NF-κB and the activation of its downstream signaling pathway in human intestinal epithelial Caco-2 cells to reduce inflammatory response[1].
Hederacoside C (5, 10, 50 μg/mL, 1 h) has anti-inflammatory effects on RAW 264.7 cells stimulated by Staphylococcus aureus[2].
Hederacoside C (5, 10 μg/mL, 2 h) has a protective effect on ECM degradation induced by senescence of mouse chondrocytes[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Caco-2
Concentration: 0.1, 1, 10 μM
Incubation Time: 1 h
Result: Reduced the expression of p-p65/p65, p-JNK, p-ERK, and p-p38.

RT-PCR[2]

Cell Line: RAW 264.7
Concentration: 5, 10, 50 μg/mL
Incubation Time: 1 h
Result: Reduced the expressions of IL-1β, IL-6, TNF-α, and IL-10.
In Vivo

Hederacoside C (0.625, 1.25, 2.5 mg/kg, intraperitoneally injected for 7 consecutive days) can alleviate TNBS-induced enteritis[1].
Hederacoside C (5, 10, 50 mg/kg, intraperitoneally injected for 3 consecutive times for 8 h) can attenuate the breast lesions caused by Staphylococcus aureus[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: TNBS-induced colitis in rat[1]
Dosage: 0.625, 1.25, 2.5 mg/kg
Administration: i.p. for 7 days
Result: Decreased the levels of inflammatory cytokines, including TNF-α, IL-6, IL-1β, CXCL-1, CXCL-2, and CXCL-5.
Reduced cell apoptosis in TNBS-induced colitis.
Reduced Bax/Bcl-2 ratio, cleaved caspase 3, and p53 protein levels in a dose-dependent manner.
Molecular Weight

1221.38

Formula

C59H96O26

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@]([H])(O[C@@H]2[C@@H](O)[C@@H](O)CO[C@]2(O[C@@H]3[C@@](C)(CO)[C@@](CC[C@]4(C)[C@]5([H])CC=C6[C@@]4(C)CC[C@]7(C(O[C@@H]8O[C@H](CO[C@H]9[C@H](O)[C@@H](O)[C@H](O[C@@]%10([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O%10)[C@@H](CO)O9)[C@@H](O)[C@H](O)[C@H]8O)=O)[C@@]6([H])CC(C)(C)CC7)([H])[C@]5(C)CC3)[H])O[C@@H](C)[C@H](O)[C@H]1O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (81.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8187 mL 4.0937 mL 8.1875 mL
5 mM 0.1637 mL 0.8187 mL 1.6375 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.05 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.35%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.8187 mL 4.0937 mL 8.1875 mL 20.4687 mL
5 mM 0.1637 mL 0.8187 mL 1.6375 mL 4.0937 mL
10 mM 0.0819 mL 0.4094 mL 0.8187 mL 2.0469 mL
15 mM 0.0546 mL 0.2729 mL 0.5458 mL 1.3646 mL
20 mM 0.0409 mL 0.2047 mL 0.4094 mL 1.0234 mL
25 mM 0.0327 mL 0.1637 mL 0.3275 mL 0.8187 mL
30 mM 0.0273 mL 0.1365 mL 0.2729 mL 0.6823 mL
40 mM 0.0205 mL 0.1023 mL 0.2047 mL 0.5117 mL
50 mM 0.0164 mL 0.0819 mL 0.1637 mL 0.4094 mL
60 mM 0.0136 mL 0.0682 mL 0.1365 mL 0.3411 mL
80 mM 0.0102 mL 0.0512 mL 0.1023 mL 0.2559 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Hederacoside C
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HY-N0253
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