1. Immunology/Inflammation
  2. Macrophage migration inhibitory factor (MIF)
  3. ISO-1

ISO-1  (Synonyms: MIF Antagonist)

Cat. No.: HY-16692 Purity: 99.94%
COA Handling Instructions

ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC50 of 7 μM.

For research use only. We do not sell to patients.

ISO-1 Chemical Structure

ISO-1 Chemical Structure

CAS No. : 478336-92-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 88 In-stock
Solution
10 mM * 1 mL in DMSO USD 88 In-stock
Solid
5 mg USD 80 In-stock
10 mg USD 130 In-stock
50 mg USD 390 In-stock
100 mg USD 620 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

Top Publications Citing Use of Products

    ISO-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.  [Abstract]

    ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.

    ISO-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.  [Abstract]

    ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC50 of 7 μM.

    IC50 & Target

    IC50: 7 μM (MIF)[1]

    In Vitro

    ISO-1 (0.1-20 μM; 16 hours) has a slight inhibitory effect on Cox-2 secretion without the addition of recombinant MIF[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[1]

    Cell Line: RAW 264.7 macrophage cells
    Concentration: 0.1μM,1μM,10μM,20μM
    Incubation Time: 16 hours
    Result: Suppressed Cox-2 secretion.
    In Vivo

    ISO-1 (injected intraperitoneally; 3.5-35 mg/kg; twice daily; 2 weeks) improves the survival rate from lethal endotoxemia and shows the anti-inflammatory effect[2].
    ISO-1 (intraperitoneal injection; 35 mg/kg; twice daily; 3 days) causes a significant reduction in average implant size and decreases Flk1 expression in implants[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57Bl/6 MIF+/+ and MIF–/– mice[2]
    Dosage: 3.5-35 mg/kg
    Administration: Injected intraperitoneally; 3.5-35 mg/kg; twice a day ; 2 weeks
    Result: Was protective against lethal sepsis.
    Animal Model: Female C57BL/6-Tg(ACTB-EGFP)1Osb/J mice[3]
    Dosage: 35 mg/kg
    Administration: Intraperitoneal injection; 35 mg/kg; twice daily; 3 days
    Result: Reduced average endometriotic implant size.
    Molecular Weight

    235.24

    Formula

    C12H13NO4

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(OC)CC1ON=C(C(C=C2)=CC=C2O)C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    Ethanol : 100 mg/mL (425.10 mM; Need ultrasonic)

    DMSO : 50 mg/mL (212.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.2510 mL 21.2549 mL 42.5098 mL
    5 mM 0.8502 mL 4.2510 mL 8.5020 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 10 mg/mL (42.51 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.94%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 4.2510 mL 21.2549 mL 42.5098 mL 106.2744 mL
    5 mM 0.8502 mL 4.2510 mL 8.5020 mL 21.2549 mL
    10 mM 0.4251 mL 2.1255 mL 4.2510 mL 10.6274 mL
    15 mM 0.2834 mL 1.4170 mL 2.8340 mL 7.0850 mL
    20 mM 0.2125 mL 1.0627 mL 2.1255 mL 5.3137 mL
    25 mM 0.1700 mL 0.8502 mL 1.7004 mL 4.2510 mL
    30 mM 0.1417 mL 0.7085 mL 1.4170 mL 3.5425 mL
    40 mM 0.1063 mL 0.5314 mL 1.0627 mL 2.6569 mL
    50 mM 0.0850 mL 0.4251 mL 0.8502 mL 2.1255 mL
    60 mM 0.0708 mL 0.3542 mL 0.7085 mL 1.7712 mL
    80 mM 0.0531 mL 0.2657 mL 0.5314 mL 1.3284 mL
    100 mM 0.0425 mL 0.2125 mL 0.4251 mL 1.0627 mL
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    ISO-1
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