1. Immunology/Inflammation
  2. SOD
  3. Imisopasem manganese

Imisopasem manganese  (Synonyms: M40403; GC4403)

Cat. No.: HY-13336 Purity: 98.15%
COA Handling Instructions

Imisopasem manganese (M40403) is a stable non-peptidyl mimetic of manganese superoxide MnSOD.

For research use only. We do not sell to patients.

Imisopasem manganese Chemical Structure

Imisopasem manganese Chemical Structure

CAS No. : 218791-21-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
USD 209 In-stock
Solution
10 mM * 1 mL in Water USD 209 In-stock
Solid
2 mg USD 119 In-stock
5 mg USD 198 In-stock
10 mg USD 317 In-stock
25 mg USD 594 In-stock
50 mg USD 977 In-stock
100 mg USD 1307 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Imisopasem manganese

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Imisopasem manganese (M40403) is a stable non-peptidyl mimetic of manganese superoxide MnSOD.

In Vitro

Imisopasem manganese is a small molecule, synthetic manganese containing superoxide dismutase mimetic (SODm) that removes superoxide anions without interfering with other reactive species known to be involved in inflammatory responses (e.g. nitric oxide, NO and peroxynitrite, ONOO-)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Imisopasem manganese is a small-molecule superoxide dismutase mimetic that has shown efficacy in animal model disease states in which superoxide anions are thought to play a key role. Imisopasem manganese inhibits the inflammatory response following the intrapleural injection of carrageenan in rats. All parameters of inflammation are attenuated by Imisopasem manganese except for NOx, PGE2 and IL-10 which remains unaltered[1]. Decreased apoptosis of the large and particularly the small bowel and marked recovery of both lymphoid and hematopoietic tissues occurs in the Imisopasem manganese pre-treated mice. Imisopasem manganese is effective in reducing TBI-induced tissue destruction and has potential as a new radioprotective agent[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

479.35

Formula

C21H31Cl2MnN5

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Cl-][Mn+2]1234([Cl-])[N]5=C6C=CC=C5CN1[C@]7([H])CCCC[C@@]7([H])N2CCN3[C@@](CCCC8)([H])[C@]8([H])N4C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 25 mg/mL (52.15 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0862 mL 10.4308 mL 20.8616 mL
5 mM 0.4172 mL 2.0862 mL 4.1723 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 8.33 mg/mL (17.38 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Animal Administration
[1][2]

Rats: Male Sprague-Dawley rats are used in the study. M40403 (5-20 mg kg-1), or an equivalent volume (0.3 mL) of vehicle (26 mm sodium bicarbonate buffer, pH 8.1-8.3), is injected intraperitoneally (i.p.) 15 min before carrageenan. At 4 h after the injection of carrageenan, the animals are killed by inhalation of CO2[1].

Mice: 30 mg dry powder M40403 is dissolved in 6.0 ml SBC adjusted to pH 8.3 with 1 M NaOH. This stock solution is diluted to 1.25 mg/mL in SBC. Two experimental models are tested. In one, the dose of IR is held constant at 8.5 Gy total body irradiation (TBI). The mice are injected i.p. with a single dose of 40 mg/kg, 30 mg/kg, 20 mg/kg or 10 mg/kg M40403. Thirty minutes later the mice receives 8.5 Gy total body irradiation (TBI). Control animals receives 0.1 ml of SBC buffer prior to TBI. In the other model, groups of 20 mice receives either 6.5 or 7.5 Gy TBI. One half of each group is treated with 2.0 mg/kg M40403 i.p. and the other with SBC 30 min before TBI. All are followed for survival[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.0862 mL 10.4308 mL 20.8616 mL 52.1540 mL
5 mM 0.4172 mL 2.0862 mL 4.1723 mL 10.4308 mL
10 mM 0.2086 mL 1.0431 mL 2.0862 mL 5.2154 mL
15 mM 0.1391 mL 0.6954 mL 1.3908 mL 3.4769 mL
20 mM 0.1043 mL 0.5215 mL 1.0431 mL 2.6077 mL
25 mM 0.0834 mL 0.4172 mL 0.8345 mL 2.0862 mL
30 mM 0.0695 mL 0.3477 mL 0.6954 mL 1.7385 mL
40 mM 0.0522 mL 0.2608 mL 0.5215 mL 1.3038 mL
50 mM 0.0417 mL 0.2086 mL 0.4172 mL 1.0431 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Imisopasem manganese Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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