2. Cell Cycle/DNA Damage
  3. Topoisomerase
  4. Indotecan

Indotecan  (Synonyms: LMP-400; NSC-724998)

Cat. No.: HY-18351 Purity: ≥98.0%
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Indotecan (LMP-400), an indenoisoquinoline derivative, is a potent Topoisomerase I inhibitor, with IC50s of 300, 1200, 560 nM for P388, HCT116, MCF-7 cell lines, respectively. Indotecan prevents the relaxation of supercoiled DNA and can be used for the research of visceral leishmaniasis.

For research use only. We do not sell to patients.

Indotecan Chemical Structure

Indotecan Chemical Structure

CAS No. : 915303-09-2

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Description

Indotecan (LMP-400), an indenoisoquinoline derivative, is a potent Topoisomerase I inhibitor, with IC50s of 300, 1200, 560 nM for P388, HCT116, MCF-7 cell lines, respectively. Indotecan prevents the relaxation of supercoiled DNA and can be used for the research of visceral leishmaniasis[1][2].

IC50 & Target[1]

Topoisomerase I

 

In Vitro

Indotecan (48 h) inhibits the proliferation of L. infantum promastigotes, ex vivo-infected splenocytes, and uninfected splenocytes, with IC50s of 0.10 μM, 0.10 μM, and 57.16 μM, respectively[2].
Indotecan (1.1-90 μM; 30 min) induces TopI-DNA complexes and inhibits DNA synthesis in L. infantum cultures[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Indotecan Related Antibodies
In Vivo

Indotecan (2.5 mg/kg; i.p. every 2 d for 15 d) depletes the parasitic burden in the spleen and liver of visceral leishmaniasis mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c mice (4-6 weeks) were infected with metacyclic parasites intravenously through the tail vein[2]
Dosage: 2.5 mg/kg body weight per injection
Administration: Intraperitoneally every 2 days for 15 days (total, eight doses)
Result: A drastic reduction of the number of transforming amastigotes recovered from the target organs of drug-treated animals was observed.
Clinical Trial
Molecular Weight

478.49

Formula

C26H26N2O7
CAS No.
Appearance

Solid

Color

White to gray

SMILES

O=C1C2=CC(OC)=C(OC)C=C2C3=C(C(C=C(OCO4)C4=C5)=C5C3=O)N1CCCN6CCOCC6
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : < 1 mg/mL (insoluble or slightly soluble)

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indotecan915303-09-2LMP-400 NSC-724998LMP400LMP 400NSC724998NSC 724998NSC-724998TopoisomeraseP388HCT116MCF-7upercoiled DNAvisceral leishmaniasisInhibitorinhibitorinhibit

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