2. NF-κB Apoptosis Autophagy Metabolic Enzyme/Protease
  3. Keap1-Nrf2 Apoptosis Autophagy Endogenous Metabolite
  4. Luteolin

Luteolin  (Synonyms: Luteoline; Luteolol; Digitoflavone)

Cat. No.: HY-N0162 Purity: 99.01%
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Luteolin (Luteoline), a flavanoid compound, is a potent Nrf2 inhibitor. Luteolin has anti-inflammatory, anti-cancer properties, including the induction of apoptosis and cell cycle arrest, and the inhibition of metastasis and angiogenesis, in several cancer cell lines, including human non-small lung cancer cells.

For research use only. We do not sell to patients.

Luteolin Chemical Structure

Luteolin Chemical Structure

CAS No. : 491-70-3

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10 mM * 1 mL in DMSO
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Based on 33 publication(s) in Google Scholar

Other Forms of Luteolin:

Luteolin Related Antibodies

Top Publications Citing Use of Products

32 Publications Citing Use of MCE Luteolin

WB

    Luteolin purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Jun 13;9:620.  [Abstract]

    Effects of Luteolin on the level of CTSD and p-CDK2 in HK-2 cells. The western blotting results of CTSD and p-CDK2 in the NaOx-stimulated HK-2 cells treated by Luteolin.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Luteolin (Luteoline), a flavanoid compound, is a potent Nrf2 inhibitor. Luteolin has anti-inflammatory, anti-cancer properties, including the induction of apoptosis and cell cycle arrest, and the inhibition of metastasis and angiogenesis, in several cancer cell lines, including human non-small lung cancer cells[1][2][3].

    In Vitro

    Luteolin (0-160 µM; 24 hours; NCI-H460 cells) treatment inhibits the viability of NCI-H460 cells in a concentration-dependent manner[1].
    Luteolin (20-80 µM; 24 hours; NCI-H460 cells) treatment causes an accumulation of cells in the S phase[1].
    Luteolin (320-580 µM; 48 hours; NCI-H460 cells) treatment induces apoptosis[1].
    Luteolin (20-80 µM; 24 hours; NCI-H460 cells) treatment increases the protein expression levels of apoptotic regulatory proteins, including the Bax/Bcl-2 ratio, in a concentration-dependent manner, however, only 80 µM Luteolin inhibits the expression of Bad. Luteolin also decreases the expression of Sirt1 in the NCI-H460 cell line in a concentration-dependent manner[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Luteolin Related Antibodies

    Cell Viability Assay[1]

    Cell Line: NCI-H460 cells
    Concentration: 0 µM, 20 µM, 40 µM, 80 µM and 160 µM
    Incubation Time: 24 hours
    Result: Inhibited the viability of NCI-H460 cells in a concentration-dependent manner.

    Cell Cycle Analysis[1]

    Cell Line: NCI-H460 cells
    Concentration: 20 µM, 40 µM, 80 µM
    Incubation Time: 24 hours
    Result: Induced cell cycle arrest in the S phase.

    Apoptosis Analysis[1]

    Cell Line: NCI-H460 cells
    Concentration: 320 µM, 440 µM, 580 µM
    Incubation Time: 48 hours
    Result: Apoptotic fraction was markedly increased.

    Western Blot Analysis[1]

    Cell Line: NCI-H460 cells
    Concentration: 20 µM, 40 µM, 80 µM
    Incubation Time: 24 hours
    Result: Increased the protein expression levels of apoptotic regulatory proteins and decreased the expression of Sirt1 in the NCI-H460 cell line in a concentration-dependent manner.
    In Vivo

    Luteolin (10-100 mg/kg; oral gavage; daily; for 12 weeks; adult male Wistar rats) has an antioxidant effect and can also protect against non-alcoholic steatohepatitis through targeting the pro-inflammatory IL-1 and Il-18 pathways in rats with a high carbohydrate/high fat diet[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Wistar rats (200-220 g)[2]
    Dosage: 10 mg/kg, 25 mg/kg, 50 mg/kg or 100 mg/kg
    Administration: Oral gavage; daily; for 12 weeks
    Result: Significantly reduced ALT and AST activity and reduced levels of bilirubin, hyaluronic acid and malondialdehyde. Shows an antioxidant activity such as a significant increase in reduced glutathione. IFN-γ, TNF-α, IL-1α and IL-18 levels decreased significantly.
    Clinical Trial
    Molecular Weight

    286.24

    Formula

    C15H10O6
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C=C(C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C13
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (349.36 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4936 mL 17.4679 mL 34.9357 mL
    5 mM 0.6987 mL 3.4936 mL 6.9871 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.27 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 20 mg/mL (69.87 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.01%

    COA (248 KB) HNMR (298 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.4936 mL 17.4679 mL 34.9357 mL 87.3393 mL
    5 mM 0.6987 mL 3.4936 mL 6.9871 mL 17.4679 mL
    10 mM 0.3494 mL 1.7468 mL 3.4936 mL 8.7339 mL
    15 mM 0.2329 mL 1.1645 mL 2.3290 mL 5.8226 mL
    20 mM 0.1747 mL 0.8734 mL 1.7468 mL 4.3670 mL
    25 mM 0.1397 mL 0.6987 mL 1.3974 mL 3.4936 mL
    30 mM 0.1165 mL 0.5823 mL 1.1645 mL 2.9113 mL
    40 mM 0.0873 mL 0.4367 mL 0.8734 mL 2.1835 mL
    50 mM 0.0699 mL 0.3494 mL 0.6987 mL 1.7468 mL
    60 mM 0.0582 mL 0.2911 mL 0.5823 mL 1.4557 mL
    80 mM 0.0437 mL 0.2183 mL 0.4367 mL 1.0917 mL
    100 mM 0.0349 mL 0.1747 mL 0.3494 mL 0.8734 mL
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    Luteolin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Luteolin491-70-3Luteoline Luteolol DigitoflavoneKeap1-Nrf2ApoptosisAutophagyEndogenous MetaboliteInhibitorinhibitorinhibit

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