2. Anti-infection
  3. Parasite Bacterial
  4. Ronidazole

Ronidazole is a potent and orally active antiprotozoal and anti-microbial agent. Ronidazole acts as a veterinary agent against Tritrichomonas foetus in cats models. Ronidazole can be used the research of forhistomoniasis and swine dysentery.

For research use only. We do not sell to patients.

Ronidazole Chemical Structure

Ronidazole Chemical Structure

CAS No. : 7681-76-7

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Based on 2 publication(s) in Google Scholar

Other Forms of Ronidazole:

Ronidazole Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ronidazole is a potent and orally active antiprotozoal and anti-microbial agent. Ronidazole acts as a veterinary agent against Tritrichomonas foetus in cats models. Ronidazole can be used the research of forhistomoniasis and swine dysentery[1][2][3].

In Vitro

Ronidazole (0.0625~0.25 μg/mL;48 hours) can effectively inhibit the growth of C. difficile all 24 strains[2].
Ronidazole (0.3125 μg/mL, 1.25 μg/mL; 8 hours) can reduce the C. difficile bacteria count to below the detection limit, and has concentration-dependent bactericidal activity against C. difficile bacteria[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ronidazole Related Antibodies

Cell Viability Assay[2]

Cell Line: C. difficile strains
Concentration:
Incubation Time: 48 hours
Result: The MICs of ronidazole ranged from 0.0625 to 0.25 μg/mL, while the MIC50 and MIC90 values were 0.125 μg/mL.

Cell Viability Assay[2]

Cell Line: C. difficile strains
Concentration: 0.3125 μg/mL, 1.25 μg/mL
Incubation Time: 8 hours
Result: Ronidazole, at 0.3125 μg/mL, reduces the initial inoculum by more than 50%. At 1.25 μg/mL, ronidazole reduced the burden of C. difficile by more than 50% in less than four hours. Bacteria did not regrow when exposed to 1.25 μg/mL of ronidazole.
In Vivo


Pharmacokinetic Analysis in six cats[3]

Dose (mg/kg) Time (h) AUClast Cmax (ng/mL)
Drug administration Dose (mg/kg) T1/2 (K) (h) C0 (μg/mL) AUC 0-∞ (h•μg/m) Vd or Vd/F (mL/kg) Clearance or CL/F (h) MRT (h) Tmax (h) Cmax (μg/m) F (%)
i.v. 9.175 9.80 13.27 187.30 700 0.82 14.14 NA NA NA
p.o. 28.23 10.50 NA 566.98 770 0.84 NA 1.02 35.37 99.64

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:
Dosage:
Administration:
Result:
Animal Model: CDI mice model
Dosage: 1 mg/kg, 10mg/kg
Administration: Oral gavage
Result: Both 1 mg/kg and 10 mg/kg Ronidazole protected 60% of mice from infection
Molecular Weight

200.15

Formula

C6H8N4O4
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

NC(OCC1=NC=C([N+]([O-])=O)N1C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (499.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4 mg/mL (19.99 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.9963 mL 24.9813 mL 49.9625 mL
5 mM 0.9993 mL 4.9963 mL 9.9925 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 5 mg/mL (24.98 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.79%

COA (244 KB) LCMS (112 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 4.9963 mL 24.9813 mL 49.9625 mL 124.9063 mL
5 mM 0.9993 mL 4.9963 mL 9.9925 mL 24.9813 mL
10 mM 0.4996 mL 2.4981 mL 4.9963 mL 12.4906 mL
15 mM 0.3331 mL 1.6654 mL 3.3308 mL 8.3271 mL
DMSO 20 mM 0.2498 mL 1.2491 mL 2.4981 mL 6.2453 mL
25 mM 0.1999 mL 0.9993 mL 1.9985 mL 4.9963 mL
30 mM 0.1665 mL 0.8327 mL 1.6654 mL 4.1635 mL
40 mM 0.1249 mL 0.6245 mL 1.2491 mL 3.1227 mL
50 mM 0.0999 mL 0.4996 mL 0.9993 mL 2.4981 mL
60 mM 0.0833 mL 0.4164 mL 0.8327 mL 2.0818 mL
80 mM 0.0625 mL 0.3123 mL 0.6245 mL 1.5613 mL
100 mM 0.0500 mL 0.2498 mL 0.4996 mL 1.2491 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ronidazole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ronidazole7681-76-7ParasiteBacterialTritrichomonas foetushistomoniasisswinedysenteryanti-microbialClostridioides difficileInhibitorinhibitorinhibit

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