2. Cell Cycle/DNA Damage Apoptosis Autophagy
  3. Eukaryotic Initiation Factor (eIF) Apoptosis Autophagy
  4. Silvestrol

Silvestrol  (Synonyms: (-)-Silvestrol)

Cat. No.: HY-13251 Purity: 98.11%
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Silvestrol is a eukaryotic translation initiation factor 4A (eIF4A) inhibitor isolated from Agave americana Linn.. Silvestrol induces autophagy and caspase-mediated apoptosis.

For research use only. We do not sell to patients.

Silvestrol Chemical Structure

Silvestrol Chemical Structure

CAS No. : 697235-38-4

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1 mg USD 840 In-stock
2 mg USD 1500 In-stock
5 mg USD 3200 In-stock
10 mg USD 4500 In-stock
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Customer Review

Based on 59 publication(s) in Google Scholar

Other Forms of Silvestrol:

Silvestrol Related Antibodies

Top Publications Citing Use of Products

42 Publications Citing Use of MCE Silvestrol

WB
IF

    Silvestrol purchased from MedChemExpress. Usage Cited in: Clin Cancer Res. 2018 Sep 1;24(17):4256-4270.  [Abstract]

    Western blot showing protein expression of translationally regulated genes in DLBCL cells treated with DMSO (D), 50 nM Silvestrol (S) or 5 μM elatol for 16h. Representative images, n=3.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2018 Sep;157:151-158.  [Abstract]

    Immunofluorescence staining of HEVp6 transfected Huh7.5 cells treated with Silvestrol (low concentration: 2.5 nM and medium concentration: 10 nM), ICN-1229 or DMSO 4 h after transfection for 42 h.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Jun 2;10(6). pii: E301.  [Abstract]

    Representative Western Blot of cellular lysates derived from cells treated with either 2 nM or 50 nM Silvestrol. DMSO treatment serves as control.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Oct 30;10(11). pii: E592.  [Abstract]

    Infection of 293T cells with CHIKV-mCherry in the presence of 50 nM silvestrol. Western blot analysis of cell lysates prepared from 293T cells infected with CHIKV-mCherry.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Mar 27;10(4). pii: E149.  [Abstract]

    A549 cells are infected with ZIKV FP and treated with the indicated amount of Silvestrol. Cell lysates of the indicated time points are analyzed by Western blot with specific antibodies against NS1 and β-actin.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2018 Mar 27;10(4). pii: E149.  [Abstract]

    A549 cells are infected with ZIKV U and treated with the indicated amount of Silvestrol. Cell lysates of the indicated time points are analyzed by Western blot with specific antibodies against NS1 and β-actin.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Cancer Biology (Medicine), University of Miami. 2018 Jul.

    Western blot analysis of translationally regulates protein expression after 16h treatment.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Oncogene. 2017 Nov 9;36(45):6225-6234.  [Abstract]

    Sin1 protein level decreases in cells treated with silvestrol. HT29-P or HCT116 cells were treated with indicated concentration of Silvestrol for 24 h. The ratio of Sin1/GAPDH and Snail/GAPDH in DMSO (0 nM Silvestrol) treated cells is designated as 1.0.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2017 Jan;137:76-81.  [Abstract]

    PIM1 levels are strongly decreased (to almost below detection limits) in the presence of 10 nM Silvestrol. This inhibitory effect persists for at least 5 days using a single dose of Silvestrol, without affecting cellular β-Actin levels.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2017 Dec 16;150:123-129.  [Abstract]

    Huh-7 cells are infected with HCoV-229E at an MOI of 3 and treated with the indicated concentrations of silvestrol. Cell lysates are prepared at 12 h p.i. and 48 h p.i. as indicated and the accumulation of viral N and nsp8 proteins is determined by Western blot analysis.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2017 Dec 16;150:123-129.  [Abstract]

    MRC-5 cells are infected with HCoV-229E at an MOI of 3 and treated with the indicated concentrations of silvestrol. Cell lysates are prepared at 12 h p.i. and 48 h p.i. as indicated and the accumulation of viral N and nsp8 proteins is determined by Western blot analysis.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Viruses. 2017 Dec 18;9(12). pii: E388.  [Abstract]

    Western blotting analysis of A549 cell lysates obtained at the indicated times post-infection with the PR8 strain of IAV and treated with 320 nM silvestrol (Sil.) or 20 nM pateamine A (PatA) at 4 hpi or the equivalent time after mock infection.

    Silvestrol purchased from MedChemExpress. Usage Cited in: EMBO J. 2016 Jun 1;35(11):1186-203.  [Abstract]

    The inhibitory effect of Silvestrol in translation is observed despite a two to five fold increase of reporter mRNA levels in HEK293 cells

    Silvestrol purchased from MedChemExpress. Usage Cited in: Cancer Discov. 2015 Jul;5(7):768-81.  [Abstract]

    Immunoblots of four colorectal cell lines upon treatment with increasing concentration of Silvestrol or solvent control (n=2).

    Silvestrol purchased from MedChemExpress. Usage Cited in: Nature. 2014 Sep 4;513(7516):65-70.  [Abstract]

    Immunoblots of lysates from human T-ALL lines treated with Silvestrol (25 nM, 24h) and probed as indicated.

    Silvestrol purchased from MedChemExpress. Usage Cited in: Blood. 2014 Dec 11;124(25):3758-67.  [Abstract]

    (A)Protein lysates from human splenic B cells are harvested 24 hours after treatment with Isotype + Vehicle, Isotype + 10 nM Silvestrol, anti-BCR + Vehicle, or anti-BCR + 10 nM Silvestrol and incubated with m7GTP Sepharose beads overnight at 4°C. Western blot analysis is used to observe eIF4A and eIF4E binding to m7GTP. Data are representative of 3e independent experiments. (B) Twenty-four hours after treatment, cells are incubated with 1 mCi L-[35S]methionine and L-[35S]cy

    Silvestrol purchased from MedChemExpress. Usage Cited in: University of Maryland, Baltimore. Molecular Medicine. 2014.

    IgG positive B-cells display more significant modulation of CARD11 protein levels upon BCR activation and treatment with Silvestrol.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Silvestrol is a eukaryotic translation initiation factor 4A (eIF4A) inhibitor isolated from Agave americana Linn.. Silvestrol induces autophagy and caspase-mediated apoptosis[1][2][3].

    IC50 & Target[3]

    eIF4

     

    In Vitro

    Silvestrol is a specific eIF4A-targeting translation inhibitor. Silvestrol exhibits significant cytotoxic activity against many human cancer cell lines, such as lung, prostate, and breast cancer with IC50 values ranging from 1 to 7 nM[1].
    ? Silvestrol significantly reduces the number of LNCaP cell colonies. Silvestrol (30 nM, 120 nM) induces apoptosis in LNCaP cells, through the mitochondrial pathway. Apaf-1, Caspase-2, caspase-9, and caspase-10 are involved in Silvestrol-induced apoptosis but caspase-3 and 7 are not[2].
    ? Silvestrol induces caspase-3 activation and apoptotic cell death in a time- and dose-dependent manner. Silvestrol-mediated cell death is attenuated in ATG7-null mouse embryonic fibroblasts (MEFs) lacking a functional autophagy protein[3].
    ? Silvestrol (50 nM) exerts an immediate inhibitory effect and causes near-static cell index compared with the control cells. Silvestrol (6.25 nM) enhances proliferation more than the vehicle control-treated cells, whereas a higher concentration of Silvestrol (50 nM) can inhibit cell proliferation. Silvestrol and episilvestrol display synergistic effects in combination with CDDP[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Silvestrol Related Antibodies
    In Vivo

    Silvestrol (1.5 mg/kg, i.p.) does not adversely affect production of human IgG by xenografted B-lymphocytes in mice. Silvestrol significantly prolongs survival compared to vehicle. There is no such lymphocyte infiltration detected in the spleens of any of the Silvestrol-treated mice, and nor do these animals exhibit any other obvious signs of lymphoma upon necropsy[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    654.66

    Formula

    C34H38O13
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C([C@H]([C@H]1C2=CC=CC=C2)[C@@H](O)[C@]3(O)[C@@]1(C4=CC=C(OC)C=C4)OC5=CC(O[C@@H]6O[C@@H]([C@H](O)CO)CO[C@H]6OC)=CC(OC)=C35)OC
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 130 mg/mL (198.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5275 mL 7.6376 mL 15.2751 mL
    5 mM 0.3055 mL 1.5275 mL 3.0550 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3.25 mg/mL (4.96 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3.25 mg/mL (4.96 mM); Clear solution

      This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References
    Cell Assay
    [2]

    The cells are seeded at a density of 7×104 cells/mL in 100-mm culture dishes and are treated with 30 nM or 120 nM concentrations of Silvestrol for 24 h[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [5]

    Mice[5]
    Peripheral blood mononuclear cells (PBMC) are injected intraperitoneally (IP) into SCID mice depleted of murine natural killer (NK) cells by pretreatment (plus weekly re-treatment) with anti-asialo (GM1). Engraftment is confirmed by hu-IgG ELISA. Treatments with vehicle (30% hydroxypropyl-β-cyclodextrin) or Silvestrol (1.5 mg/kg every 48 hr IP) begin 2 weeks post-engraftment[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5275 mL 7.6376 mL 15.2751 mL 38.1878 mL
    5 mM 0.3055 mL 1.5275 mL 3.0550 mL 7.6376 mL
    10 mM 0.1528 mL 0.7638 mL 1.5275 mL 3.8188 mL
    15 mM 0.1018 mL 0.5092 mL 1.0183 mL 2.5459 mL
    20 mM 0.0764 mL 0.3819 mL 0.7638 mL 1.9094 mL
    25 mM 0.0611 mL 0.3055 mL 0.6110 mL 1.5275 mL
    30 mM 0.0509 mL 0.2546 mL 0.5092 mL 1.2729 mL
    40 mM 0.0382 mL 0.1909 mL 0.3819 mL 0.9547 mL
    50 mM 0.0306 mL 0.1528 mL 0.3055 mL 0.7638 mL
    60 mM 0.0255 mL 0.1273 mL 0.2546 mL 0.6365 mL
    80 mM 0.0191 mL 0.0955 mL 0.1909 mL 0.4773 mL
    100 mM 0.0153 mL 0.0764 mL 0.1528 mL 0.3819 mL
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    Silvestrol Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Silvestrol697235-38-4(-)-SilvestrolEukaryotic Initiation Factor (eIF)ApoptosisAutophagyInhibitorinhibitorinhibit

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