Toltrazuril (sulfone) (Synonyms: Ponazuril)

Cat. No.: HY-17008 Purity: 98.58%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Toltrazuril sulfone (Ponazuril) is a metabolite of Toltrazuril (HY-B0175), with antiprotozoal activity. Toltrazuril sulfone is a triazine anticoccidial that is developed to prevent coccidiosis in poultry.

For research use only. We do not sell to patients.

Toltrazuril (sulfone) Chemical Structure

CAS No. : 69004-04-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 125	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
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Other Forms of Toltrazuril (sulfone):

Toltrazuril In-stock

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Coccidia

In Vitro

Toltrazuril sulfone inhibits the development of merozoites of S. neurona^[1].
Toltrazuril sulfone inhibits the development of N. caninum after approximately 48 h post-exposure^[1].
Toltrazuril sulfone exhibits inhibitory possibly by targeting different enzyme/enzyme systems in different apicomplexans^[1].
Toltrazuril sulfone (5 mg/ml; 20 hours) inhibits T. gondii replication after the second division by endodyogeny^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Toltrazuril sulfone (10-20 mg/kg; p.o.; daily; for 10 days) is effective in preventing and treating toxoplasmosis in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female CD-1 mice^[2]
Dosage:	10 mg/kg, 20 mg/kg
Administration:	Oral administration, daily, for 10 days
Result:	Prevented and protected mice from toxoplasmosis.

Molecular Weight

457.38

Formula

C₁₈H₁₄F₃N₃O₆S

CAS No.

69004-04-2

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(C=C1)OC2=C(C=C(C=C2)N3C(N(C(NC3=O)=O)C)=O)C)(C(F)(F)F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (109.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1864 mL	10.9318 mL	21.8637 mL
5 mM	0.4373 mL	2.1864 mL	4.3727 mL
10 mM	0.2186 mL	1.0932 mL	2.1864 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.58%

Select Batch:

Data Sheet (274 KB) SDS (0 KB)

COA (249 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Sheila M Mitchell, et al. The effects of ponazuril on development of apicomplexans in vitro. J Eukaryot Microbiol. May-Jun 2005;52(3):231-5. [Content Brief]

[2]. Sheila M Mitchell, et al. Efficacy of ponazuril in vitro and in preventing and treating Toxoplasma gondii infections in mice. J Parasitol. 2004 Jun;90(3):639-42. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1864 mL	10.9318 mL	21.8637 mL	54.6591 mL
	5 mM	0.4373 mL	2.1864 mL	4.3727 mL	10.9318 mL
	10 mM	0.2186 mL	1.0932 mL	2.1864 mL	5.4659 mL
	15 mM	0.1458 mL	0.7288 mL	1.4576 mL	3.6439 mL
	20 mM	0.1093 mL	0.5466 mL	1.0932 mL	2.7330 mL
	25 mM	0.0875 mL	0.4373 mL	0.8745 mL	2.1864 mL
	30 mM	0.0729 mL	0.3644 mL	0.7288 mL	1.8220 mL
	40 mM	0.0547 mL	0.2733 mL	0.5466 mL	1.3665 mL
	50 mM	0.0437 mL	0.2186 mL	0.4373 mL	1.0932 mL
	60 mM	0.0364 mL	0.1822 mL	0.3644 mL	0.9110 mL
	80 mM	0.0273 mL	0.1366 mL	0.2733 mL	0.6832 mL
	100 mM	0.0219 mL	0.1093 mL	0.2186 mL	0.5466 mL

Toltrazuril (sulfone) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Toltrazuril (sulfone)69004-04-2PonazurilParasiteToltrazurilanticoccidialantiprotozoalInhibitorinhibitorinhibit

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