Oct-4 Antibody

Oct-4 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 39 kDa, targeting to POU5F1. It can be used for WB,IHC-P,ICC/IF,IP,ChIP assays with tag free, in the background of Human, Mouse.

POU5F1: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Free

5460

Predicted band size: 39 kDa;Observed band size: 45 kDa

affinity purified

Non-conjugated

Epigenetics and Nuclear Signaling

Primary Antibody; Rabbit Polyclonal Antibody

Rabbit

Human, Mouse

Q01860

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;IP: 1:20 ;ChIP: 1:20

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  Western blot analysis of extracts from Hela(lane 2(10μg) or (lane 3(20μg)), using PKC gamma (HY-P80770) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80770, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, IP, ChIP

Liquid

Supplied in phosphate buffered saline (pH 7.4), 150 mM NaCl and 50% glycerol. Preservative: 0.02% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human Oct4.The exact sequence is proprietary to MCE.