Phospho-Hormone sensitive lipase (Ser853) Antibody

Cat. No.: HY-P80464: FAQs
Data Sheet SDS COA User Guide for Antibodies

Phospho-Hormone sensitive lipase (Ser853) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 117 kDa, targeting to Phospho-Hormone sensitive lipase (S853). It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
Free Sample		Apply now
10 μL	$70	In-stock	Estimated Time of Arrival: December 31
50 μL	$185	In-stock	Estimated Time of Arrival: December 31
100 μL	$300	In-stock	Estimated Time of Arrival: December 31
250 μL		Get quote

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Biological Activity
Technical Parameters
Properties
Documentation

Description	Phospho-Hormone sensitive lipase (Ser853) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 117 kDa, targeting to Phospho-Hormone sensitive lipase (S853). It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse, Rat.
Tag	Free
Synonyms	Hormone sensitive lipase antibody; Hormone sensitive lipase testicular isoform antibody; Hormone-sensitive lipase antibody; HSL antibody; LHS; Lipase hormone sensitive antibody; LIPE antibody; LIPS_HUMAN antibody; Phospho-Hormonesensitivelipase(S853); PhosphoHormonesensitivelipase(S853)
Molecular Weight	Predicted band size: 117 kDa;Observed band size: 90 kDa
Purity	Protein A affinity purified.
Subcellular Location	Cell membrane, cytosol, caveola, Lipid droplet.
Conjugation	Non-conjugated
Research Field	Cardiovascular
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Host	Rabbit
Reactivity	Human, Mouse, Rat
SwissProt ID	SwissProt: Q05469 Human;SwissProt: P54310 Mouse;SwissProt: P15304 Rat
Dilution Ratio	WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 Western blot analysis of extracts from NIH3T3(lane 2(20μg) or lane 3(40μg)) and 3T3-L1(lane 4(20μg) or lane 5(40μg)), using Phospho-Hormone sensitive lipase (Ser853) (HY-P80464) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80464, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Application	WB, IHC-P
Appearance	Liquid
Formulation	Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Immunogen	Synthetic phosphopeptide corresponding to residues surrounding Ser853 of Human LIPE.AA range:821-870.
Documentation	Select Batch: Data Sheet (231 KB) SDS (251 KB) COA (206 KB) User Guide for Antibodies (1077 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Hormone sensitive lipase (Ser853) Antibody

Biological Activity

Technical Parameters

Properties

Documentation

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