Autophagy inducer 3

Name: Autophagy inducer 3
Brand: MedChemExpress
SKU: HY-146052
Rating: 4.5 (1 reviews)

Cat. No.: HY-146052 Purity: 99.23%: FAQs
Data Sheet SDS COA Handling Instructions

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Autophagy inducer 3 has autophagy induced activity. Autophagy inducer 3 possesses robust autophagic cell death in diverse cancer cells sparing normal counterpart. Autophagy inducer 3 induces lethal autophagy by formation of characteristic autophagic vacuoles, LC3 puncta formation, upregulation of signature autophagy markers like Beclin and Atg family proteins.

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Autophagy inducer 3 Chemical Structure

CAS No. : 2691054-63-2

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Autophagy^[1]

In Vitro

Autophagy inducer 3 (compound 26b) (10 μM; 48 hours) effectively inhibits cell growth in diverse breast, lung and colon cancer cell lines^[1].
Autophagy inducer 3 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in COLO-205, LOVO, HT-29, DLD-1, SW48 and SW-620 with IC₅₀s of 2.03 μM, 3.33 μM, 4.15 μM, 4.46 μM, 3.14 μM, 1.86 μM, respectively; and shows low cytotoxicity in normal human colon fibroblast CCD-18Co with IC₅₀ over 10 μM^[1].
Autophagy inducer 3 (7.5 μM; 18 hours) promotes non apoptotic cell death in DLD-1 cells by cellular granularity through vacuole formation and increase Annexin-V positive cells as well as PARP cleavage; and robustly induces the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	MCF-7, MDA-MB-231, DLD-1, HT-29, A549 and NCI-H358^[1]
Concentration:	10 μM
Incubation Time:	48 hours
Result:	Effectively inhibited cell growth at 10 μM dose in diverse cancer cell lines with the inhibition rates of 89.28~97.66%.

Cell Autophagy Assay

Cell Line:	DLD-1^[1]
Concentration:	7.5 μM
Incubation Time:	18 hours
Result:	Promoted intracytoplasmic vacuole accumulation in colon cancer (DLD-1) cells; and robustly induced the expression of classical autophagy markers like Beclin-1, Atg3, Atg5 and Atg7.

Molecular Weight

377.60

Formula

C₂₄H₄₃NO₂

CAS No.

2691054-63-2

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCC[C@H](O)[C@@H](N)CC1=CC=C(C=C1)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (264.83 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6483 mL	13.2415 mL	26.4830 mL
5 mM	0.5297 mL	2.6483 mL	5.2966 mL
10 mM	0.2648 mL	1.3242 mL	2.6483 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: 2.5 mg/mL (6.62 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.23%

Select Batch:

Data Sheet (268 KB) SDS (251 KB)

COA (240 KB) HNMR (115 KB) RP-HPLC (153 KB) LCMS (227 KB)

Handling Instructions (2659 KB)

References

[1]. Ganesher A, et al. New Spisulosine Derivative promotes robust autophagic response to cancer cells. Eur J Med Chem. 2020;188:112011. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.6483 mL	13.2415 mL	26.4831 mL	66.2076 mL
	5 mM	0.5297 mL	2.6483 mL	5.2966 mL	13.2415 mL
	10 mM	0.2648 mL	1.3242 mL	2.6483 mL	6.6208 mL
	15 mM	0.1766 mL	0.8828 mL	1.7655 mL	4.4138 mL
	20 mM	0.1324 mL	0.6621 mL	1.3242 mL	3.3104 mL
	25 mM	0.1059 mL	0.5297 mL	1.0593 mL	2.6483 mL
	30 mM	0.0883 mL	0.4414 mL	0.8828 mL	2.2069 mL
	40 mM	0.0662 mL	0.3310 mL	0.6621 mL	1.6552 mL
	50 mM	0.0530 mL	0.2648 mL	0.5297 mL	1.3242 mL
	60 mM	0.0441 mL	0.2207 mL	0.4414 mL	1.1035 mL
	80 mM	0.0331 mL	0.1655 mL	0.3310 mL	0.8276 mL
	100 mM	0.0265 mL	0.1324 mL	0.2648 mL	0.6621 mL