2. C16-Ceramide

C16-Ceramide is a natural small molecule activating p53 through the direct and selective binding.

For research use only. We do not sell to patients.

C16-Ceramide Chemical Structure

C16-Ceramide Chemical Structure

CAS No. : 24696-26-2

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Based on 1 publication(s) in Google Scholar

Other Forms of C16-Ceramide:

C16-Ceramide Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE C16-Ceramide

  • Biological Activity

  • Purity & Documentation

  • Customer Review

Description

C16-Ceramide is a natural small molecule activating p53 through the direct and selective binding[1].

IC50 & Target

p53[1], Apoptosis[2]
In Vitro

C16-Ceramide interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide in the cell[1].
C16-Ceramide (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner[2].
C16-Ceramide (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells[2].
C16-ceramide (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-ceramide down-regulates Mdm2 expression via Btf[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C16-Ceramide Related Antibodies

Cell Viability Assay[2]

Cell Line: HCT116 cells
Concentration: 2.5, 5, 10, 12, 20, 50 µM
Incubation Time: 0-48 h
Result: Strongly decreased cell viability in a time- and concentration-dependent manner.

Western Blot Analysis[2]

Cell Line: HCT116 cells
Concentration: 12 μM
Incubation Time: 1, 3 and 6 h
Result: Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion also partially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.
Molecular Weight

537.90

Formula

C34H67NO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCCC(N[C@@H](CO)[C@H](O)/C=C/CCCCCCCCCCCCC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMF : 20 mg/mL (37.18 mM; ultrasonic and warming and heat to 60°C)

Ethanol : 20 mg/mL (37.18 mM; warming and heat to 60°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8591 mL 9.2954 mL 18.5908 mL
5 mM 0.3718 mL 1.8591 mL 3.7182 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    90% Corn Oil

    Solubility: ≥ 2 mg/mL (3.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

COA (237 KB) Elemental Analysis Report (96 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF / Ethanol 1 mM 1.8591 mL 9.2954 mL 18.5908 mL 46.4770 mL
5 mM 0.3718 mL 1.8591 mL 3.7182 mL 9.2954 mL
10 mM 0.1859 mL 0.9295 mL 1.8591 mL 4.6477 mL
15 mM 0.1239 mL 0.6197 mL 1.2394 mL 3.0985 mL
20 mM 0.0930 mL 0.4648 mL 0.9295 mL 2.3239 mL
25 mM 0.0744 mL 0.3718 mL 0.7436 mL 1.8591 mL
30 mM 0.0620 mL 0.3098 mL 0.6197 mL 1.5492 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C16-Ceramide24696-26-2Inhibitorinhibitorinhibit

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