2. Enzyme
  3. Tool Enzyme

Tool Enzyme

There are many types of tool enzymes with different functions, including common polymerases, reverse transcriptases, modifying enzymes, etc.

Tool enzymes are mainly used for:

• Molecular biology research

• mRNA synthesis

Tool Enzyme (18):

Cat. No. Product Name CAS No.  
  • HY-E70090
    T7 RNA polymerase 9014-24-8  
    T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter.
  • HY-E70099
    Poly(A) polymerase 9026-30-6  
    Poly(A) polymerase catalyzes the incorporation of ATP into the RNA 3' end in the form of AMP, that is, adding a polyadenosine tail at the RNA 3' end. Poly(A) polymerase increases RNA stability and increases mRNA translation efficiency in eukaryotic cells. Poly(A) polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA.
  • HY-131161
    M-MLV Reverse transcriptase 9068-38-6  
    M-MLV Reverse transcriptase (M-MLV RT) is a kind of Reverse transcriptase, from the moroni mouse leukemia virus (MoMLV).
  • HY-E70080
    Vaccinia virus capping enzyme  
    Vaccinia virus capping enzyme is a transcription initiation factor. Vaccinia virus capping enzyme is a heterodimer of D1 (844 aa) and D12 (287 aa) polypeptides that executes all three steps in m7GpppRNA synthesis. Vaccinia virus capping enzyme has been used widely as a reagent for capping and cap-labeling RNAs in vitro.
  • HY-E70081
    mRNA Cap 2'-O-methyltransferase  
    mRNA Cap 2'-O-methyltransferase uses S-adenosylmethionine (SAM) as a methyl donor to add a methyl group at the 2'-O position of the first nucleotide at the 5’ end of Cap-0 mRNA, resulting in Cap-1 structure. Cap-1 structure promotes translation efficiency, increasing subsequent protein expression.
  • HY-E70217
    Uracil DNA Glycosylase (UDG/UNG), heat-sensitive 59088-21-0  
    Uracil DNA Glycosylase (UDG/UNG), heat-sensitive (Heat-sensitive UDG) can catalyze the hydrolysis of the N-glycosidic bond between the uracil base and the sugar phosphate backbone in the DNA chain containing uracil, releasing free uracil. Uracil DNA Glycosylase (UDG/UNG), heat-sensitive eliminates the carryover contamination dUTP-incorporated amplification products.
  • HY-E70086A
    Taq DNA Polymerase, Glycerol-free 2304873-37-6  
    Taq DNA Polymerase, Glycerol-free is a thermostable DNA polymerase that can be used in PCR.
  • HY-E70216
    Bsu DNA polymerase, Large fragment  
    Bsu DNA polymerase, Large fragment is a polymerase derived from Bacillus subtilis. Bsu DNA polymerase, Large fragment is a DNA isothermal amplification polymerase with chain replacement activity, which used in RPA recombinase polymerase amplification technology.
  • HY-P2937
    DNA polymerase 9012-90-2  
    DNA polymerase is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
  • HY-P3005
    T4 DNA ligase 9015-85-4  
    DNA ligase is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
  • HY-E70084
    Tth DNA polymerase 2409055-60-1  
    Tth DNA polymerase is a DNA polymerase from T. thermophilus that can be used for DNA sequencing and polymerase chain reaction (PCR).
  • HY-E70085
    Moloney murine leukemia virus RT  
    Moloney murine leukemia virus RT is a monomeric reverse transcriptase from Moloney murine leukemia virus (MMLV). Moloney murine leukemia virus RT is a replicative polymerase that play an essential role in the life cycle of the retrovirus.
  • HY-E70086
    Taq DNA polymerase 2304873-37-6  
    Taq DNA polymerase is a thermostable DNA polymerase that can be used in PCR.
  • HY-E70094
    T4 UvsX Recombinase  
    T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates.
  • HY-E70095
    T4 UvsY Protein  
    T4 UvsY Protein is an accessory protein for in vitro catalysis of strand exchange. T4 UvsY Protein enhances strand exchange by UvsX protein by interacting specifically with UvsX protein. UvsY protein enhances the rate of single-stranded-DNA-dependent ATP hydrolysis by UvsX protein.
  • HY-E70091
    T4 RNA ligase  
    T4 RNA ligase is an ATP-dependent RNA ligase that can catalyze the formation of phosphodiester bonds between the 5'-P end and the 3'-OH end of single-stranded RNA, single-stranded DNA or single nucleotides between molecules or within molecules. T4 RNA ligase is used for enzymatic low ribonucleotide synthesis and 3′ end labeling of RNA. It is mainly used for RNA and RNA, but can also be used for RNA and single nucleotides.
  • HY-E70232
    Asparagine-tRNA ligase 37211-76-0  
    Asparagine-tRNA ligase is an enzyme that catalyzes the chemical reaction. Asparagine-tRNA ligase belongs to the ligase family. Asparagine-tRNA ligase is involved in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.
  • HY-E70233
    Histidyl-tRNA synthetase, human 9068-78-4  
    Histidyl-tRNA synthetase, human is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. Histidyl-tRNA synthetase has been found to act as a particularly important antigen in autoimmune diseases such as rheumatic arthritis or myositis.