Ethoxysanguinarine

Name: Ethoxysanguinarine
Brand: MedChemExpress
SKU: HY-N4317
Rating: 4.5 (1 reviews)

Cat. No.: HY-N4317 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ethoxysanguinarine is a benzophenanthridine alkaloid natural product that is mainly found in Macleaya cordata. Ethoxysanguinarine is an inhibitor of protein phosphatase 2A (CIP2A). Ethoxysanguinarine induces cell apoptosis and inhibits colorectal cancer cells growth.

For research use only. We do not sell to patients.

Ethoxysanguinarine Chemical Structure

CAS No. : 28342-31-6

Size	Price	Stock	Quantity
1 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Ethoxysanguinarine

•Cancer Lett. 2020 Oct 7;496:57-71. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Target: CIP2A, Apoptosis^[1]

In Vitro

Ethoxysanguinarine inhibits cells vialibity with IC₅₀ of 9.37 μM (SW480 cells); 7.19 μM (HCT116 cells); 6.55 μM (HT69 cells); 3.57 μM (SW620 cells)^[1].
Ethoxysanguinarine (3 μM or 5 μM, 24 h) down-regulates phospho-Akt, decreases CIP2A/PP2A/Akt pathway expression in the SW620 and HT29 cells^[1].
Ethoxysanguinarine (3 μM or 5 μM, 24 h) increases the PP2A activity and inhibits phosphorylation of CIP2A downstream molecule protein kinase B^[1].
Ethoxysanguinarine (1-3 μM or 4.5-5 μM, 24 h) decreases CIP2A transcription (protein level) and induces CIP2A proteolysis in the SW620 and HT29 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Colorectal cancer (CRC) cell lines: SW620, SW480, HT29 and HCT116
Concentration:	0.5-12 μM
Incubation Time:	24 hours
Result:	Inhibited the viability of CRC cells with IC₅₀ of 3.57-9.37 μM.

Apoptosis Analysis^[1]

Cell Line:	Colorectal cancer (CRC) cell lines: SW620, HT29
Concentration:	0-3 μM or 0-5 μM, respectively
Incubation Time:	24 hours
Result:	Induced caspase-dependent apoptosis in colorectal cancer (CRC) cells.

In Vivo

Ethoxysanguinarine (0.5 mg/kg; s.c.; 5 times per week; 4 weeks) represses colorectal cancer (CRC) xenograft tumors growth in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nude immunodeficient mice (5-week-old, weighing about 16 g)^[1]
Dosage:	0.5 mg/kg
Administration:	Subcutaneous injection; 5 times per week for 4 weeks
Result:	Exhibited antitumor effects without reduce the body weight of the mice.

Molecular Weight

377.39

Formula

C₂₂H₁₉NO₅

CAS No.

28342-31-6

Appearance

Solid

Color

White to yellow

SMILES

CN1C2=C3C(C=C4OCOC4=C3)=CC=C2C5=C(C6=C(OCO6)C=C5)C1OCC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 60 mg/mL (158.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6498 mL	13.2489 mL	26.4978 mL
5 mM	0.5300 mL	2.6498 mL	5.2996 mL
10 mM	0.2650 mL	1.3249 mL	2.6498 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.51 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (280 KB) SDS (251 KB)

COA (253 KB) HNMR (265 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Jin L, et al. Ethoxysanguinarine inhibits viability and induces apoptosis of colorectal cancer cells by inhibiting CIP2A. Int J Oncol. 2018 Mar 16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6498 mL	13.2489 mL	26.4978 mL	66.2445 mL
	5 mM	0.5300 mL	2.6498 mL	5.2996 mL	13.2489 mL
	10 mM	0.2650 mL	1.3249 mL	2.6498 mL	6.6244 mL
	15 mM	0.1767 mL	0.8833 mL	1.7665 mL	4.4163 mL
	20 mM	0.1325 mL	0.6624 mL	1.3249 mL	3.3122 mL
	25 mM	0.1060 mL	0.5300 mL	1.0599 mL	2.6498 mL
	30 mM	0.0883 mL	0.4416 mL	0.8833 mL	2.2081 mL
	40 mM	0.0662 mL	0.3312 mL	0.6624 mL	1.6561 mL
	50 mM	0.0530 mL	0.2650 mL	0.5300 mL	1.3249 mL
	60 mM	0.0442 mL	0.2208 mL	0.4416 mL	1.1041 mL
	80 mM	0.0331 mL	0.1656 mL	0.3312 mL	0.8281 mL
	100 mM	0.0265 mL	0.1325 mL	0.2650 mL	0.6624 mL