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Incomplete Freund's adjuvant (IFA)  (Synonyms: Montanide ISA-51)

Cat. No.: HY-153808A
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Incomplete Freund's adjuvant (IFA) (Montanide ISA-51) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund. Incomplete Freund's adjuvant (IFA) does not contain inactivated tuberculosis bacilli and consists of petroleum jelly containing lanolin. Incomplete Freund's adjuvant (IFA) induces high antibody titers and long-lasting effector T cell responses with no long-term effects on collagen disease, tumors, or death. Complete Freund's adjuvant (CFA) (HY-153808) is another type of Freund's Adjuvant that stimulates a stronger immune response.

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Incomplete Freund's adjuvant (IFA) Chemical Structure

Incomplete Freund's adjuvant (IFA) Chemical Structure

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10 mL USD 30 In-stock
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5*10 mL USD 100 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Incomplete Freund's adjuvant (IFA) (Montanide ISA-51) is an immunoadjuvant emulsified with antigen by its discoverer Jules T. Freund. Incomplete Freund's adjuvant (IFA) does not contain inactivated tuberculosis bacilli and consists of petroleum jelly containing lanolin. Incomplete Freund's adjuvant (IFA) induces high antibody titers and long-lasting effector T cell responses with no long-term effects on collagen disease, tumors, or death. Complete Freund's adjuvant (CFA) (HY-153808) is another type of Freund's Adjuvant that stimulates a stronger immune response[1][2].

In Vivo

Incomplete Freund's adjuvant (IFA) can be used in animal modeling to establish a mouse model of persistent inflammatory pain. Incomplete Freund's adjuvant (IFA) induces antibody responses, while Complete Freund's adjuvant (CFA) (HY-153808) preferentially induces autoantigen-specific responses[2].
Complete Freund's adjuvant (CFA) has low plasma clearance (22%)[4].
1. Induction of experimental autoimmune myocarditis (EAM)[1]
Background
Complete Freund's adjuvant (CFA) induces myocarditis by activating the immune system and enhancing the antigen-specific immune response.
Specific Mmodeling Methods
Mice: A/J, BALB/c, and IL6KO BALB/c • male • 6-8-week-old
Administration: 5mg/ml • s.c. • twice
Note
(1) On day 0, all mice additionally received 500 ng of pertussis toxin (HY-112779) (i.p.)
(2) On days 0 and 7, mice received subcutaneous injections peptide emulsified in CFA supplemented with 5 mg/mL of Mycobacterium tuberculosis, strain H37Ra.


Modeling Indicators
Histological analysis: Myocarditis severity was assessed by histology and relative heart weight.
Molecular changes: Test serum levels of total IgG, IgG1, IgG2a, IgG2b, IL-1b and IL6.
Correlated Product(s): Incomplete Freund's adjuvant (IFA) (HY-153808A)
Opposite Product(s):
2. Induction of Neuropathic Pain[2][3]
Background
Complete Freund's adjuvant (CFA) releases inflammatory mediators TNF-α and IL-6 in body which can activate peripheral nerve endings and act on pain nerve endings, resulting in neuropathic pain[2][3].
Specific Mmodeling Methods
Mice: C57BL/6J • male • 8-week-old
Administration: 20 μl • s.c. • once
Note
(1) Inject Complete Freund’s Adjuvant (CFA) without dilution into the plantar area of the hind paw.
(2) Tests PWT and PWL for both hind paws corresponding to before and 24, 48, 72 h after CFA injection.


Modeling Indicators
Behavior Observation:Detect paw withdrawal threshold (PWT) and paw withdrawal latency (PWL).
Correlated Product(s): Galantamine (HY-76299)
Opposite Product(s): Mecamylamine (HY-B1395A)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Incomplete Freund's adjuvant (IFA)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

COA (240 KB)

Handling Instructions (2659 KB)
References
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Incomplete Freund's adjuvant (IFA) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Incomplete Freund's adjuvant (IFA)Montanide ISA-51Montanide ISA51Montanide ISA 51Othersimmunologic adjuvantimmunityinflammationimmunization-induced animal modelscollagen-induced arthritisCIAexperimental autoimmune encephalomyelitisEAEexperimental autoimmune thyroiditisEATexperimental autoimmune uveitisEAUexperimental autoimmune myocarditisEAMInhibitorinhibitorinhibit

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