Streptavidin Agarose 6FF

Cat. No.: HY-K0218A: FAQs
Manual SDS

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

Size	Price	Stock	Quantity
Free Sample (1 mL)		Apply Now
5 mL	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mL	USD 530	In-stock	Estimated Time of Arrival: December 31
50 mL	USD 2340	In-stock	Estimated Time of Arrival: December 31

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Streptavidin Agarose 6FF Featured Recommendations:

Description
Storage
Components
Documentation

Description
& Advantages

Streptavidin, isolated from Streptomyces avidinii, binds 4 moles of biotin per mole of protein with an extremely high affinity. With no carbohydrate group and an isoelectric point of 6.5 (different from avidin), streptavidin has less nonspecific binding. Streptavidin has a strong affinity with biotin and needs to be eluted under denaturing conditions when purified. However, streptavidin has a relatively weak affinity for iminobiotin, which binds at pH 9.5-11.0 and elutes at pH 4.0 without the use of denaturing agents.

Characteristics
Matrix Spherical	6% cross-linked agarose
Ligand	Streptavidin
Bead Diameter	>> 45-165 μm
Binding Capacity	>200 nmol of D-Biotin/mL Settled Resin
Maximum Pressure	0.3 MPa, 3 bar
pH	4.0-9.0

Publications

•Chem Biol Interact. 2023 Aug 28, 110681.

Storage

2-8°C, 2 years. Do not dry or freeze.

Components

Components	HY-K0218A-5 mL	HY-K0218A-10 mL	HY-K0218A-50 mL
Streptavidin Agarose 6FF	5 mL	10 mL	50 mL

Documentation

Manual (155 KB) SDS (130 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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