2. Academic Validation
  3. The synthetic antimicrobial peptide pexiganan and its nanoparticles (PNPs) exhibit the anti-helicobacter pylori activity in vitro and in vivo

The synthetic antimicrobial peptide pexiganan and its nanoparticles (PNPs) exhibit the anti-helicobacter pylori activity in vitro and in vivo

  • Molecules. 2015 Mar 2;20(3):3972-85. doi: 10.3390/molecules20033972.
Xiao-Lin Zhang 1,  An-Min Jiang 2,  Zhong-You Ma 3,  Xian-Bao Li 4,  You-Yi Xiong 5,  Jin-Feng Dou 6,  Jian-Fei Wang 7 8
Affiliations

Affiliations

  • 1 The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China. [email protected].
  • 2 The School of Life Science, University of Science and Technology of China, Hefei 230032, China. [email protected].
  • 3 The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China. [email protected].
  • 4 The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China. [email protected].
  • 5 The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China. [email protected].
  • 6 The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China. [email protected].
  • 7 The Department of Pharmacy, Food and Drug School, Anhui Science and Technology University, Fengyang 233100, China. [email protected].
  • 8 The Ministry of Agriculture Key Laboratory of Microbial Organic Fertilizer, Bengbu 233030, China. [email protected].
PMID: 25738539 DOI: 10.3390/molecules20033972
Abstract

The aim of this study was to probe the potential anti-H. pylori activity of the synthetic antimicrobial peptide pexiganan, which is an analog of the peptide magainin, and its nanoparticles (PNPs) that were prepared in our laboratory. To compare their Antibacterial effects in vitro and in vivo, studies of H. pylori growth inhibition, kinetics and resistance assays were undertaken. The gastric mucoadhesive efficiency and H. pylori clearance efficiency of pexiganan and PNPs were evaluated in rats and mice infected with H. pylori. The eradication of H. pylori was determined using urease tests and a microbial culture method. We observed that PNPs adhered to gastric mucosa more effectively owing to a prolonged stay in the stomach, which resulted in a more effective H. pylori clearance. In addition, PNPs had greater anti-H. pylori effect than pexiganan in infected mice. The amount of pexiganan required to eradicate H. pylori was significantly less using PNPs than the corresponding pexiganan suspension. The results confirmed that PNPs improved peptide stability in the stomach and more effectively eradicated H. pylori from mice stomachs than pexiganan.

Figures
Products