2. Academic Validation
  3. Ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppressing nicotinic acetylcholine receptor-ion channels in cultured bovine adrenal medullary cells

Ikarisoside A inhibits acetylcholine-induced catecholamine secretion and synthesis by suppressing nicotinic acetylcholine receptor-ion channels in cultured bovine adrenal medullary cells

  • Naunyn Schmiedebergs Arch Pharmacol. 2015 Dec;388(12):1259-69. doi: 10.1007/s00210-015-1161-y.
Xiaojia Li 1,  Yumiko Toyohira 1,  Takafumi Horisita 2,  Noriaki Satoh 3,  Keita Takahashi 1,  Han Zhang 4,  Munekazu Iinuma 5,  Yukari Yoshinaga 1,  Susumu Ueno 6,  Masato Tsutsui 7,  Takeyoshi Sata 2,  Nobuyuki Yanagihara 8
Affiliations

Affiliations

  • 1 Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan.
  • 2 Department of Anesthesiology, University of Occupational and Environmental Health, School of Medicine, Yahatanishi-ku, Kitakyushu, Japan.
  • 3 Shared-Use Research Center, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Japan.
  • 4 Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
  • 5 Department of Pharmacognosy, Gifu Pharmaceutical University, Daigakunishi, Gifu, Japan.
  • 6 Department of Occupational Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu, Japan.
  • 7 Department of Pharmacology, Faculty of Medicine, University of The Ryukyus, Nishihara, Okinawa, Japan.
  • 8 Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu, 807-8555, Japan. [email protected].
PMID: 26257152 DOI: 10.1007/s00210-015-1161-y
Abstract

Ikarisoside A is a natural flavonol glycoside derived from Plants of the genus Epimedium, which have been used in Traditional Chinese Medicine as tonics, antirheumatics, and aphrodisiacs. Here, we report the effects of ikarisoside A and three other flavonol glycosides on Catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. We found that ikarisoside A (1-100 μM), but not icariin, epimedin C, or epimedoside A, concentration-dependently inhibited the secretion of catecholamines induced by acetylcholine, a physiological secretagogue and agonist of nicotinic acetylcholine receptors. Ikarisoside A had little effect on Catecholamine secretion induced by veratridine and 56 mM K(+). Ikarisoside A (1-100 μM) also inhibited (22)Na(+) influx and (45)Ca(2+) influx induced by acetylcholine in a concentration-dependent manner similar to that of Catecholamine secretion. In Xenopus oocytes expressing α3β4 nicotinic acetylcholine receptors, ikarisoside A (0.1-100 μM) directly inhibited the current evoked by acetylcholine. It also suppressed (14)C-catecholamine synthesis and tyrosine hydroxylase activity induced by acetylcholine at 1-100 μM and 10-100 μM, respectively. The present findings suggest that ikarisoside A inhibits acetylcholine-induced Catecholamine secretion and synthesis by suppression of nicotinic acetylcholine receptor-ion channels in bovine adrenal medullary cells.

Keywords

Adrenal medulla; Catecholamine secretion; Epimedium; Flavonoids; Ikarisoside A; Nicotinic acetylcholine receptor.

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